Fluo-4 acetoxymethyl ester (Fluo-4 AM) is a cell-permeable fluorescent calcium indicator.{57160} It is cleaved by intracellular esterases to release Fluo-4, which binds to calcium with a Kd value of 345 nM and displays excitation/emission maxima of 490/
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A cell-permeable fluorescent calcium indicator; cleaved by intracellular esterases to release Fluo-4, which binds to calcium (Kd = 345 nM); ex/em = 490/520 nm, respectively; has been used to measure intracellular calcium levels in live cells by fluorescent microscopy or flow cytometry