Z-VDVAD-AFC is a fluorogenic substrate for caspase-2.{47223} Upon enzymatic cleavage by caspase-2, 7-amino-4-trifluoromethylcoumarin (AFC) is released and its fluorescence can be used to quantify caspase-2 activity. AFC displays excitation/emission maxi
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A fluorogenic substrate for caspase-2; AFC is released upon enzymatic cleavage by caspase-2 and its fluorescence can be used to quantify caspase-2 activity; ex/em = 400/505 nm, respectively