Ac-DNLD-AMC is a fluorogenic caspase-3 substrate.{39492} Upon enzymatic cleavage by caspase-3, 7-amino-4-methylcoumarin (AMC) is released and its fluorescence can be used to quantify caspase-3 activity. AMC displays excitation/emission maxima of 340-360
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A fluorogenic caspase-3 substrate; upon enzymatic cleavage by caspase-3, AMC is released and its fluorescence can be used to quantify caspase-3 activity; AMC ex/em = 340-360/440-460 nm, respectively