Goat anti-Mouse Albumin cross-

（共享）ELISA原理与实验方法

, incubate for 30 min at 37°C. ( 5g powdered milk in 95 ml PBS)5 Rinse with PBS 3x.6 Add 50 µl of antibody, incubate 30 min at 37°C.7 Rinse with PBS 3x.8 Add 50 µl of goat anti-mouse IgG conjugate with alkaline phosphatase.(1 µl of conjugate in 4 ml of PBS

Anti-HA Immunocytology

-purified goat anti-mouse IgG (Jackson Labs; 1 mg/ml stock used at 1:300 dilution) Texas Red-conjugated affinity-purified donkey anti-rabbit (Jackson Labs;1 mg/ml stock used at 1:200 dilution)  

Isolation and Purification Method of Mouse Fetal Hepatoblasts

developed by our group to isolate mouse fetal hepatoblasts (MFHs) with high purity. The method is based on a selective harvesting of the hepatic parenchymal cells from fetuses (E 14.5), followed by the sorting of E-cadherin+ progenitors through the use