Biotinylated Human Nectin-2 (C-6His-Avi) (CY73)
产品说明(Description)
Biotinylated Recombinant Human Poliovirus Receptor-Related Protein 2 is produced by our Mammalian expression system and the target gene encoding Gln32-Leu360 is expressed with a Avi, 6His tag at the C-terminus.
Accession #: Q92692-2
Known as: Poliovirus Receptor-Related Protein 2; Herpes Virus Entry Mediator B; Herpesvirus Entry Mediator B; HveB; Nectin-2; CD112; PVRL2; HVEB; PRR2
制剂(Formulation)
Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.
质量控制(Quality Control)
Purity: Greater than 95% as determined by reducing SDS-PAGE.
Endotoxin: Less than 0.1 ng/µg (1 EU/µg) as determined by LAL test.
Biotin:Protein Ratio The biotin to protein ratio is 0.5-1 as determined by the HABA assay.
Bioactivity-Elisa: |
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Immobilized Human PVRIG-mFc(Cat#C08Y) at 10μg/ml (100 μl/well) can bind Biotinylated Human Nectin-2-His-Avi(Cat#CY73). The ED50 of Biotinylated Human Nectin-2-His-Avi(Cat#CY73) is 0.42 ug/ml. |
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Immobilized Human PVRIG-Fc(Cat#C09U) at 10μg/ml (100 μl/well) can bind Biotinylated Human Nectin-2-His-Avi(Cat#CY73). The ED50 of Biotinylated Human Nectin-2-His-Avi(Cat#CY73) is 0.21 ug/ml. |
Bioactivity-BLI:
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Loaded Biotinylated Human Nectin-2-His(Cat#CY73) on His Biosensor, can bind Human PVRIG(Cat#C09U) with an affinity constant of 168.5n M as determined in BLI assay. |
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Loaded Biotinylated Human Nectin-2-His(Cat#CY73) on His Biosensor, can bind Human PVRIG(Cat#C08Y) with an affinity constant of 84.68n M as determined in BLI assay. |
复溶(Reconstitution)
Always centrifuge tubes before opening. Do not mix by vortex or pipetting.
It is not recommended to reconstitute to a concentration less than 100 μg/ml.
Dissolve the lyophilized protein in distilled water.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
保存(Storage)
Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks.
Reconstituted protein solution can be stored at 4-7°C for 2-7 days.
Aliquots of reconstituted samples are stable at < -20°C for 3 months.
背景(Background)
CD112 is a type I transmembrane glycoprotein belonging to the Immunoglobulin superfamily. It comprises one Ig-like V-type domain and two Ig-like C2-type domains in the extracellular region. The V domain is believed to mediate nectin binding to its ligands. Nectin2 is known to bind the pseudorabies virus, and herpes simplex virus2 (HSV2), involving in cell to cell spreading of these viruses. It does not bind poliovirus. As a homophilic adhesion molecule, CD112 is found concentrated in adherens junctions, and exists on neurons, endothelial cells,epithelial cells and fibroblasts. CD112 has been identified as the ligand for DNAM-1 (CD226), and the interaction of CD226/CD112 mediates cytotoxicity and cytokine secretion by T and NK cells. The costimulatory responses may be a critical component in allergic reactions and may therefore become targets for anti-allergic therapy.
电泳(SDS-PAGE)
FOR RESEARCH USE ONLY