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文献和实验PEAS Labeling Gcn4-cys James Fishburn, Hahn Lab March 2006 Notes 10 mCi 125I = 4.6 nmol (see spec. sheet for calculation) 4.6 nmol Gcn4(1-118,209-281) = 103 microgram (M.W. 22,400) PEAS has low solubility in aqueous
Solid-Phase Synthesis of Neuropeptides by Fmoc Strategies
an automated peptide synthesizer that performs solid-phase peptide synthesis using the Fmoc strategy (1 ). This is based on the sequential addition of amino acid residues to an insoluble polymeric support. The base-labile Fmoc group
Difference Gel Electrophoresis Based on Lys/Cys Tagging
Before separation, proteins of different biological samples are labeled with different fluorescent dyes, the CyDye™ DIGE Fluors. Currently three dyes with spectrally different excitation and emission wavelengths are available. This allows
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