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- 详细信息
- 文献和实验
- 技术资料
- 抗体名:
山羊抗小鼠
- 抗体英文名:
Goat Anti-Mouse IgG2a 20842-250uL
- 靶点:
Mouse IgG2
- 浓度:
2 mg/mL
- 应用范围:
ELISA, Flow cytometry, IHC, IF (cells or tissue sections), Western blot
- 宿主:
Goat
- 保质期:
6个月
- 目录编号:
20842-250uL
- 库存:
99
- 供应商:
Biotium
- 克隆性:
多克隆
- 保存条件:
-20°C
- 形态:
Liquid: PBS/50% glycerol/2 mg/mL BSA/0.05% azide, Lyophilized: PBS/15 mg/mL BSA/20 mg/mL trehalose after reconstitution
This is a goat anti-mouse IgG2a isotype-specific secondary antibody labeled with our bright and photostable CF® dyes and biotin. The conjugates are prepared from affinity-purified antibodies that react with Fc portion of the heavy chain of mouse IgG2a. To minimize cross reactivity, the antibodies are cross-adsorbed against other mouse IgG subclasses (IgG1, IgG2b, IgG3), and human, bovine and rabbit serum proteins.
- Cross-adsorbed for specific staining with minimal background
- Available in 13 bright and photostable CF® dyes
- Biotin conjugate also available
- Suitable for western, immunofluorescence, and immunohistology
Note: Conjugates of blue fluorescent dyes like CF®350 and CF®405S are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
Goat Anti-Mouse IgG2a
| Conjugation | Ex/Em | Size | Catalog No. |
|---|---|---|---|
| CF®350 | 347/448 nm | 50 uL (100 ug) | 20255-1 |
| 0.25 mL (500 ug) | 20255 | ||
| CF®405S | 404/431 nm | 50 uL (100 ug) | 20381-50uL |
| 0.25 mL (500 ug) | 20381-250uL | ||
| CF®488A | 490/515 nm | 50 uL (100 ug) | 20256-1 |
| 0.25 mL (500 ug) | 20256 | ||
| CF®543 | 541/560 nm | 50 uL (100 ug) | 20326-1 |
| 0.25 mL (500 ug) | 20326 | ||
| CF®555 | 555/565 nm | 50 uL (100 ug) | 20257-1 |
| 0.25 mL (500 ug) | 20257 | ||
| CF®568 | 562/583 nm | 50 uL (100 ug) | 20258-1 |
| 0.25 mL (500 ug) | 20258 | ||
| CF®594 | 593/614 nm | 50 uL (100 ug) | 20259-1 |
| 0.25 mL (500 ug) | 20259 | ||
| CF®633 | 630/650 nm | 50 uL (100 ug) | 20260-1 |
| 0.25 mL (500 ug) | 20260 | ||
| CF®640R | 642/662 nm | 50 uL (100 ug) | 20261-1 |
| 0.25 mL (500 ug) | 20261 | ||
| CF®647 | 650/665 nm | 50 uL (100 ug) | 20262-1 |
| 0.25 mL (500 ug) | 20262 | ||
| CF®680 | 681/698 nm | 50 uL (100 ug) | 20263-1 |
| 0.25 mL (500 ug) | 20263 | ||
| CF®680R | 680/701 nm | 50 uL (100 ug) | 20842-50uL |
| 0.25 mL (500 ug) | 20842-250uL | ||
| CF®770 | 770/797 nm | 50 uL (100 ug) | 20264-1 |
| 0.25 mL (500 ug) | 20264 | ||
| Biotin | N/A | 50 uL (100 ug) | 20472-50uL |
| 0.25 mL (500 ug) | 20472-250uL |
View our full selection of bright and specific Secondary Antibodies, or search our catalog using our Antibody Finder. Alternatively, you can view our secondary antibody product listings with catalog numbers.
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文献和实验高效气相色谱仪测定人血浆中硝酸异山梨酯浓度的色谱条件: 1、仪器:高效气相色谱仪 2、检测器:ECD 3、色谱柱:石英毛细管柱 OV-101(25m×0.25 mm I.D.,膜厚 0.22um Shimadzu) 4、载气:高纯氮气 5、载气流速:1.25 mL/min 6、分流比:1:80 7、尾吹气流速:20 mL/min 8、进样口温度:200℃ 9、检测器温度:250℃ 10、程序升温:起始柱温 150℃,保持 9 min
二抗广义上是指专门和一抗进行特异性反应和结合的抗体,在免疫学反应中,经常需要针对试验选择不同的二抗,艾美捷能为您的科研工作提供最适合和最全面的二抗产品。检测任何目的靶蛋白都有不止一种抗体可供选择,同时在后继试验中也会有不同的检测方案,因此在选择二抗的时候要综合考虑一抗的类型及后继检测方案的要求,一般来说,选择合适的二抗需要从下面几个方面考虑:【一抗的种属来源】二抗应选用与使用的一抗相同的物种来源,例如:如果你的一抗是小鼠源的单克隆抗体,二抗则选抗小鼠的二抗(山羊抗小鼠或者兔抗小鼠等均
的大角野山羊抗鼠一刺猬抗体,提示在免疫染色中是标记的抗鼠―刺猬的一抗。 第二抗体是针对其他种属免疫球蛋白的抗体,即抗抗体,通常称为抗免疫球蛋白的抗体。如用一个正式的名称免疫球蛋白来描述免疫原,则用口语化的名词抗抗体来命名其产品。如果产生的抗体只针对免疫球蛋白的一个亚群,应在免疫原中加以说明。例如,羊抗鼠免疫球蛋白IgG2a抗体是指羊产生的抗鼠纯化的IgG2a抗体。
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