Human mesenchymal stem cells (MSC) are a population of multipotent cells that can be differentiated into multiple lineage-specific cells, which can form bone, fat, cartilage, muscle and tendon. The process of chondrogenesis that forms cartilage is regarded as a promising alternative to improve the quality of cartilage regeneration in repairing cartilage damage. ScienCell has created a convenient multiplex PCR kit for the routine detection of human mesenchymal stem cell chondrogenesis. Multiplex PCR allows two or more primers to be amplified in a single PCR reaction by using multiple primer pairs in a single reaction mixture, allowing for considerable saving in labor, cost and precious DNA samples. All required PCR reagents are supplied in this kit. Simply add DNA template and perform PCR reaction. The ready-PCR mix contains SOX9 and COL2A1 primers that allow for the detection of early-to-mid- and late-stage of human MSC chondrogenesis, respective [1].
Product Use
HMSC-C PCR kit is for research use only. Not for use in animals, humans, or diagnostic procedures.
Storage
Store at -20°C upon receipt. Avoid repeated freeze thaw cycles by making six aliquots at 110 μL each.
Shipping
Dry ice.
Reference
[1] Sekiya, I., Vuoristo, J. T., Larson, B. L., & Prockop, D. J. (2002). In vitro cartilage formation by human adult stem cells from bone marrow stroma defines the sequence of cellular and molecular events during chondrogenesis. PNAS, 99(7), 4397-4402.