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- 详细信息
- 文献和实验
- 技术资料
- 供应商:
GeneTex
- 检测方法:
ELISA, Sandwich ELISA
- 应用:
ELISA, Sandwich ELISA
- 样本:
Recombinant proteins
- 规格:
1 pair
Sandwich ELISA analysis of pig IgG protein using GTX03035 Mouse anti-Pig IgG antibody [MT421] as coating antibody and GTX03036-02 Mouse anti-Pig IgG antibody [MT424] (Biotin) as detecting antibody.
Substrate : TMB
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文献和实验Xenoantibodies and Complement Activity Determinations in Pig-to-Primate Xenotransplantation
total endogenous complement activity; the cytolytic activity of anti-pig antibodies (APA) is also measured by hemolytic assay; an ELISA is used for quantification of anti-galactose α1,3galactose (Gal) IgG and IgM antibodies and flow cytometry
, dilute the total 1:1 with PMG, and add MgGTP to 0.2 mM to promote MT polymerization. Disburse into 31.5 ml ultracentrifuge tubes and pair them by weight. Immerse the portion of the tubes containing the cytosol in a 37oC water bath and allow MT
collect the cytosolic supernatants from the tubes with a pipette, pool them in a graduated cylinder, dilute the total 1:1 with PMG, and add MgGTP to 0.2 mM to promote MT polymerization. Disburse into 31.5 ml ultracentrifuge tubes and pair them by weight
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