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文献和实验Use of Channelrhodopsin for Activation of CNS Neurons
on all ends. It splits light evenly and is customizable. (B ) A fiber optic rotary joint (commutator) from Doric Lenses with FC/PC connections on either end. It has minimal torque and can be easily rotated by mice. (C ) A fiber optic patch cable with rotary
In Situ HYBRIDIZATION TO TISSUE SECTIONS
dechorionated embryos to either buffer A or B (depending on their age): Buffer A (0-2, 1-3 hr embryos) 2.5 ml 8% paraformaldehyde (fresh) 2.0 ml 1X PBS 0.5 ml DMSO - or - Buffer B (2-4, 3-5
【交流】染色体免疫沉淀技术详细步骤-young lab !!!
100 mM 5M NaCl 1.0 ml 1 mM 0.5M EDTA 50.0 µl 0.5 mM 0.5M EGTA 100.0 µl 11% 37% Formaldehyde 14.9 ml ddH2O 31.5 ml II. Preblock and binding of antibody to magnetic beads NOTE: Exact type of Dynal bead (Protein A, Protein G, sheep
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