Recombinant Human CD27/TNFRSF7 (CX60)
产品说明(Description)
Recombinant Human CD27 is produced by our Mammalian expression system and the target gene encoding Thr21-Ile192 is expressed with a Fc, 6His tag at the C-terminus.
Accession #: P26842
Known as: CD27 antigen;CD27L receptor; T-cell activation antigen CD27; Tumor necrosis factor receptor superfamily member 7; T14 and TNFRSF7.
制剂(Formulation)
Lyophilized from a 0.2 μm filtered solution of 20mM Tris,150mM NaCl, pH 8.0.
质量控制(Quality Control)
Purity: Greater than 95% as determined by reducing SDS-PAGE.
Endotoxin: Less than 0.1 ng/ug (1 EU/ug) as determined by LAL test.
Elisa: Immobilized Human TNFSF7/CD70 (Cat:CI06) at 10μg/ml (100 μl/well) can bind biotinylated Human CD27/TNFRSF7 (Cat:CD60), The ED50 of Human CD27/TNFRSF7(Cat:CD60) is 1.72 ug/ml .
复溶(Reconstitution)
Always centrifuge tubes before opening. Do not mix by vortex or pipetting.
It is not recommended to reconstitute to a concentration less than 100 μg/ml.
Dissolve the lyophilized protein in distilled water.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
保存(Storage)
Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks.Reconstituted protein solution can be stored at 4-7°C for 2-7 days.Aliquots of reconstituted samples are stable at < -20°C for 3 months.
背景(Background)
CD27 antigen is also known as CD27L receptor, T-cell activation antigen CD27, Tumor necrosis factor receptor superfamily member 7, T14 and TNFRSF7. In humans, it is encoded by the CD27 gene. CD27 is a single-pass type I membrane protein with 3 TNFR-Cys repeats. It is a member of the TNF-receptor superfamily and is required for generation and long-term maintenance of T cell immunity. It binds to ligand CD70, and plays a key role in regulating B-cell activation and immunoglobulin synthesis. It plays a role in survival of activated T-cells and apoptosis through association with SIVA1.
电泳(SDS-PAGE)
FOR RESEARCH USE ONLY