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AJE 润色服务限时 8 折起,权威机构信任 15+ 年Cytoscape 教程来了!快速实现顶刊同款通路网络图五大应用案例:Mustang Q 膜层析应用全解析1 个小工具,一次性搞定流程图、质粒图谱和信号通路图- 详细信息
- 文献和实验
- 技术资料
- 抗体名:
APC标记羊抗人IgG
- 抗体英文名:
Goat Anti-human IgG/APC
- 靶点:
细胞浆 细胞膜 分泌型蛋白
- 浓度:
1mg/ml
- 应用范围:
Elisa=1:500-1000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500,ICC=1:100-500,
- 宿主:
Rabbit
- 适应物种:
Human,Mouse,Rat,Dog,Pig,Cow,Rabbit,Sheep,Guinea Pig,
- 保质期:
一年
- 抗原来源:
Rabbit
- 目录编号:
TF21166R
- 级别:
I级
- 库存:
10
- 供应商:
晶风生物
- 标记物:
FITC/Alexa/CY357/BIo/HRP
- 克隆性:
Polyclonal
- 保存条件:
-20
- 形态:
Liquid
- 亚型:
IgG
- 免疫原:
KLH conjugated synthetic peptide derived
- 规格:
100ul
产品规格:100ul/200ul(部分有50ul,如需更大包装或其他具体规格,请咨询客服)
研究领域:肿瘤 细胞生物 免疫学等
抗体来源:Rabbit
克隆类型:Polyclonal
交叉反应:Human,Mouse,Rat,Dog,Pig,Cow,Rabbit,Sheep,
产品应用:WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 Flow-Cyt=1μg/Test IF=1:100-500 (石蜡切片需做抗原修复)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
性 状:Liquid
浓 度:1mg/ml
免 疫 原:KLH conjugated synthetic peptide derived
亚 型:IgG
纯化方法:affinity purified by Protein A
储 存 液:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件:Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
APC标记羊抗人IgG相关抗体示例(非本抗体,如需本抗体,请联系客服索要说明书):
Sample:
Liver (Mouse) Lysate at 40 ug
Spleen (Mouse) Lysate at 40 ug
NIH/3T3 (Mouse) CellLysate at 30 ug
RAW246.7 (Mouse) CellLysate at 30 ug
Primary: Anti- IL12 at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 22 kD
Observed band size: 35/36 kD
paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (IL12) Polyclonal Antibody, Unconjugated at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) instructions and DAB staining.

paraffin embedded (rat brain tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (IL12) Polyclonal Antibody, Unconjugated at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) instructionsand DAB staining.

APC标记羊抗人IgG
Blank control (blue line): Mouse spleen (blue).
Primary Antibody (green line): Rabbit Anti- IL12 antibody
Dilution: 1μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody (white blue line): Goat anti-rabbit IgG-FITC
Dilution: 1μg /test.
Protocol
The cells were fixed with 70% ice-cold methanol overnight at 4℃. Cells stained with Primary Antibody for 30 min at room temperature. The cells were then incubated in 1 X PBS/2%BSA/10% goat serum to block non-specific protein-protein interactions followed by the antibody for 15 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.

Tissue/cell: rat colitis tissue; paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,at 37℃ for 20 min;
Incubation: Anti-IL-12 Polyclonal Antibody, Unconjugated 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB staining
欢迎新老客户咨询订购:APC标记羊抗人IgG
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文献和实验光染料的强度:PE > APC > FITC > PerCP。 3. 多色流式细胞检测中荧光染料荧光图案的选择原则 1) 根据机器配置选择最亮的荧光染料 了解你使用的流式细胞仪的性能,大多数流式细胞仪有两个或者多个激光器,有五种波长可供选择:紫外(355 nm)、紫色(405 nm)、蓝色(488 nm)、黄色(561 nm)和红色(640 nm)。除了激光器,具体的虑光片和检测器也决定了你的配置可同时检测多少种颜色。 多色标记荧光染料尽量选用不同激光激发的染料。荧
Nature 三连发:竞争消除,有你没我,论癌细胞的「霸道行为」
结直肠癌 (CRC) 是胃肠道中常见的恶性肿瘤,其形成也是一个逐步发展的过程。前期研究数据表明,大多数结直肠癌起始于异常的腺窝,后逐渐演变成息肉,最终发展为结肠直肠癌,整个过程可长达 5 - 15 年。在内在分子机制层面,大多数结直肠癌是通过 WNT 通路的永久激活而启动的,而这种激活通常是发生在肠道干细胞中肿瘤抑制基因 APC 的突变之后。 APC 的突变可诱导肠内细胞增殖增加,防止细胞死亡并阻断分化,所有这些特征均可能有助于提高 APC 突变细胞的相对适应度,APC 突变的肠道干细胞及其后
流式细胞术是分析细胞或颗粒特征常用的技术:将细胞表面或胞内、核内的抗原标记上荧光抗体,荧光素被激发光激发时产生荧光,从而被检测。 DAPI 即 4',6-二脒基-2-苯基吲哚(4',6-diamidino-2-phenylindole),是一种可以透过完整的细胞膜,与DNA强力结合的荧光染料,常用于荧光显微镜观测。当 DAPI 与双股 DNA 结合时,在紫外光的激发下,发出蓝光。而红细胞无细胞核(无 DNA),因此不会被 DAPI 染色。 在免疫
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