- ￥1040
- 晶风生物
- TF-0764P
- 中国
- 2025年07月12日
- Elisa=1:500-1000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500,ICC=1:100-500,
- Rabbit
- Human,Mouse,Rat,Dog,Pig,Cow,Rabbit,Sheep,Guinea Pig,
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- 详细信息
- 文献和实验
- 技术资料
- 抗体名：
胆囊收缩素/缩胆囊素(八肽)多肽
- 抗体英文名：
CCK8
- 靶点：
细胞浆 细胞膜 分泌型蛋白
- 浓度：
1mg/ml
- 应用范围：
Elisa=1:500-1000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500,ICC=1:100-500,
- 宿主：
Rabbit
- 适应物种：
Human,Mouse,Rat,Dog,Pig,Cow,Rabbit,Sheep,Guinea Pig,
- 保质期：
一年
- 抗原来源：
Rabbit
- 目录编号：
TF21166R
- 级别：
I级
- 库存：
10
- 供应商：
晶风生物
- 标记物：
FITC/Alexa/CY357/BIo/HRP
- 克隆性：
Polyclonal
- 保存条件：
-20
- 形态：
Liquid
- 亚型：
IgG
- 免疫原：
KLH conjugated synthetic peptide derived
- 规格：
500ug
产品规格：100ul/200ul(部分有50ul，如需更大包装或其他具体规格，请咨询客服)
研究领域：肿瘤 细胞生物 免疫学等
抗体来源：Rabbit
克隆类型：Polyclonal
交叉反应：Human,Mouse,Rat,Dog,Pig,Cow,Rabbit,Sheep,
产品应用：WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 Flow-Cyt=1μg/Test IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
性 状：Liquid
浓 度：1mg/ml
免 疫 原：KLH conjugated synthetic peptide derived
亚 型：IgG
纯化方法：affinity purified by Protein A
储 存 液：0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件：Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
胆囊收缩素/缩胆囊素(八肽)多肽相关抗体示例（非本抗体，如需本抗体，请联系客服索要说明书）：
Sample:
Liver (Mouse) Lysate at 40 ug
Spleen (Mouse) Lysate at 40 ug
NIH/3T3 (Mouse) CellLysate at 30 ug
RAW246.7 (Mouse) CellLysate at 30 ug
Primary: Anti- IL12 at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 22 kD
Observed band size: 35/36 kD
paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (IL12) Polyclonal Antibody, Unconjugated at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) instructions and DAB staining.
paraffin embedded (rat brain tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (IL12) Polyclonal Antibody, Unconjugated at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) instructionsand DAB staining.
胆囊收缩素/缩胆囊素(八肽)多肽
Blank control (blue line): Mouse spleen (blue).
Primary Antibody (green line): Rabbit Anti- IL12 antibody
Dilution: 1μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody (white blue line): Goat anti-rabbit IgG-FITC
Dilution: 1μg /test.
Protocol
The cells were fixed with 70% ice-cold methanol overnight at 4℃. Cells stained with Primary Antibody for 30 min at room temperature. The cells were then incubated in 1 X PBS/2%BSA/10% goat serum to block non-specific protein-protein interactions followed by the antibody for 15 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
Tissue/cell: rat colitis tissue; paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,at 37℃ for 20 min;
Incubation: Anti-IL-12 Polyclonal Antibody, Unconjugated 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB staining
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文献和实验的多肽文库。他们用一个两色“多肽文库免疫印迹色谱分析”（PELICAN）技术来探测合成出的多肽文库顺序。Sebestyten F等亦提出了发展有色多肽和多肽文库的方法。Mery J等研究了二硫键作为Boc-Bzl SPPS 的多肽-树脂接头，认为这种方法具有生物化学上的应用潜力。直接将多肽偶联在活性蛋白载体上，可能应用于抗体的产生。 其它方面，Lloyd-Williams P等用了一个光不稳定的树脂，合成了一个完全被保护的八肽，此肽包含一个Met残基，他们指出在用光裂解时，此Met可以避免被氧化
多肽与靶标结合模式的预测 分子动力学稳定上述复合物结构 多肽与靶标亲和能力强弱的预测 （3）改造多肽结构 虚拟氨基酸突变提高多肽与靶标的亲和力 分子动力学的精确计算 案例分析一、ACE 多肽抑制剂作用机理的研究 [3] 血管紧张素转化酶（angiotensin converting enzyme，ACE）是一种锌离子依赖型羧二肽酶，可以催化水解十肽血管紧张素 I 羧基末端两个氨基酸，生成具有血管收缩作用的八肽血管紧张素 II
造影，进行胰腺肿瘤或囊肿的诊断。 胆囊收缩素 也称促胰酶素，是上部小肠、十二指肠分泌的三十三肽酰胺（简称CCK－33）。其主要生理功能是刺激胆囊收缩使之分泌胆汁，并促进胰酶分泌。油类食物为胆囊收缩素的刺激物。其第27位酪氨酸残基经硫酯化后才呈现充分的生物活性。人工合成的羧端八肽硫酯物（CCK-8）即具有充分的促胆囊收缩活性。脑内也有CCK－8，是引起饱感和参与食欲调节的活性肽之一。 肠动素 为二十二肽，分布于小肠、十二指肠等处可增强胃肠蠕动；肠抑胃肽（GIP ）为四
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