Recombinant Human FGL1 (C-mFc) (CW62)
产品说明(Description)
Recombinant Human Fibrinogen-like Protein 1 is produced by our Mammalian expression system and the target gene encoding Leu23-Ile312 is expressed with a mFc tag at the C-terminus.
Accession #: Q08830
Known as:Fibrinogen-like protein 1; FGL1; HP-041; Hepassocin; HFREP-1; LFIRE-1
制剂(Formulation)
Lyophilized from a 0.2 μm filtered solution of PBS, 5% Thehalose, pH 7.4.
质量控制(Quality Control)
Purity: Greater than 95% as determined by reducing SDS-PAGE.
Endotoxin: Less than 0.1 ng/ug (1 EU/ug) as determined by LAL test.
Bioactivity: Immobilized Human LAG-3 (Cat#CU29) at 5μg/ml (100 μl/well) can bind Human FGL1-Fc (Cat#CW62).
The ED50 of Human FGL1-Fc (Cat#CW62) is 2.1 ug/ml.
复溶(Reconstitution)
Always centrifuge tubes before opening. Do not mix by vortex or pipetting.
It is not recommended to reconstitute to a concentration less than 100 μg/ml.
Dissolve the lyophilized protein in distilled water.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
保存(Storage)
Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks.
Reconstituted protein solution can be stored at 4-7°C for 2-7 days.
Aliquots of reconstituted samples are stable at < -20°C for 3 months.
背景(Background)
Hepassocin, also known as hepatocyte-derived fibrinogen-related protein 1 (HFREP-1), is a liver-specific secreted protein belonging to the fibronogen superfamily, whose members share a fibrinogen domain at their C-termini. It is secreted by the liver and functions as a mitogen for hepatocytes. Hepassocin may play a role in the development of hepatocellular carcinomas. Hepassocin is a disulfide-linked homodimeric protein with a C-terminal fibrinogen domain. It is reported that it is a major immune inhibitory ligand of LAG-3 .
电泳(SDS-PAGE)
FOR RESEARCH USE ONLY.