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- 详细信息
- 文献和实验
- 技术资料
- 抗体名:
趋化样因子受体1抗体
- 抗体英文名:
CMKLR1
- 靶点:
细胞浆 细胞膜 分泌型蛋白
- 浓度:
1mg/ml
- 应用范围:
Elisa=1:500-1000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500,ICC=1:100-500,
- 宿主:
Rabbit
- 适应物种:
Human,Mouse,Rat,Dog,Pig,Cow,Rabbit,Sheep,Guinea Pig,
- 保质期:
一年
- 抗原来源:
Rabbit
- 目录编号:
TF10183R
- 级别:
I级
- 库存:
10
- 供应商:
晶风生物
- 标记物:
FITC/Alexa/CY357/BIo/HRP
- 克隆性:
Polyclonal
- 保存条件:
-20
- 形态:
Liquid
- 亚型:
IgG
- 免疫原:
KLH conjugated synthetic peptide derived
- 规格:
50ul/100ul/200ul
产品规格:100ul/200ul(部分有50ul,如需更大包装或其他具体规格,请咨询客服)
研究领域:肿瘤 细胞生物 免疫学等
抗体来源:Rabbit
克隆类型:Polyclonal
交叉反应:Human,Mouse,Rat,Dog,Pig,Cow,Rabbit,Sheep,
产品应用:WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 Flow-Cyt=1μg/Test IF=1:100-500 (石蜡切片需做抗原修复)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
性 状:Liquid
浓 度:1mg/ml
免 疫 原:KLH conjugated synthetic peptide derived
亚 型:IgG
纯化方法:affinity purified by Protein A
储 存 液:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件:Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
CMKLR1趋化样因子受体1抗体相关抗体示例(非本抗体,如需本抗体,请联系客服索要说明书):
Sample:
Liver (Mouse) Lysate at 40 ug
Spleen (Mouse) Lysate at 40 ug
NIH/3T3 (Mouse) CellLysate at 30 ug
RAW246.7 (Mouse) CellLysate at 30 ug
Primary: Anti- IL12 at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 22 kD
Observed band size: 35/36 kD
paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (IL12) Polyclonal Antibody, Unconjugated at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) instructions and DAB staining.

paraffin embedded (rat brain tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (IL12) Polyclonal Antibody, Unconjugated at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) instructionsand DAB staining.

CMKLR1趋化样因子受体1抗体
Blank control (blue line): Mouse spleen (blue).
Primary Antibody (green line): Rabbit Anti- IL12 antibody
Dilution: 1μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody (white blue line): Goat anti-rabbit IgG-FITC
Dilution: 1μg /test.
Protocol
The cells were fixed with 70% ice-cold methanol overnight at 4℃. Cells stained with Primary Antibody for 30 min at room temperature. The cells were then incubated in 1 X PBS/2%BSA/10% goat serum to block non-specific protein-protein interactions followed by the antibody for 15 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.

Tissue/cell: rat colitis tissue; paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,at 37℃ for 20 min;
Incubation: Anti-IL-12 Polyclonal Antibody, Unconjugated 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB staining
欢迎新老客户咨询订购:CMKLR1趋化样因子受体1抗体
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文献和实验实说明两点:一是细胞因子(包括趋化因子)受体能否正常地表达,不是一件可有可无的事,有时甚至可决定一个个体是否会感染致命性的疾病;第二,是否能有效地和受体结合,是趋化因子发挥作用的关键。 趋化因子受体和HⅣ感染 A.表达受体CD4和OZR5的T细胞可被HIV感染;B.趋化因子可阻断HIV与C12R5的结合HW不能与突变的CCR5结合,不再感染T细胞。 还有一个例子是关于过敏性疾病。近来有资料表明,Thl/Tcl和Th2/Tc2亚群分别表达CXCR
、TNF-cJ即进入成熟阶段,表型和功能均发生改变:高表达MHCⅡ类分子、共刺激分子和黏附分子(如ICAM-1、DC-SIGN),不表达FcR、CR和病原体受体。虽然摄取和加工抗原的能力降低,却具有强大的提呈抗原能力,主要分泌ILq2、IL-4等细胞因子。 未成熟DC摄取抗原后发生迁移,通过输入淋巴管进入局部淋巴结,并分化为成熟DC,提呈抗原激发T细胞免疫应答。DC在体内迁移是其分化成熟和实现抗原提呈功能所必需,是DC的重要特征,与其趋化因子受体表达谱有关。未成熟DC表达CCRl、CCR
和黏附,参与了风湿性关节炎、肝炎、肺炎及移植耐受等过程。 2.CC型 又称臼趋化因子亚家族,结构特征为第1、第2半胱氨残基酸紧密相连。该亚家族的基因大多数位于入染色:体17qll―32或小鼠第11号染色体,含有2个内含子和3个外显子。CC趋化因子主要作用于单核细胞和淋巴细胞,这和CXC型亚家族成员主要作用于中性粒细胞不同。CC趋化因子还促进其他类型细胞的游走趋化,如淋巴细胞、DC、NK细胞、嗜酸粒细胞、嗜碱粒细胞等。该亚家族的代表成员是单核细胞趋化蛋白(MCP-1,),功能是使单核细胞离开
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