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- 详细信息
- 文献和实验
- 技术资料
- 抗体名:
卷曲螺旋结构域蛋白90A抗体
- 抗体英文名:
CCDC90A
- 靶点:
细胞浆 细胞膜 分泌型蛋白
- 浓度:
1mg/ml
- 应用范围:
Elisa=1:500-1000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500,ICC=1:100-500,
- 宿主:
Rabbit
- 适应物种:
Human,Mouse,Rat,Dog,Pig,Cow,Rabbit,Sheep,Guinea Pig,
- 保质期:
一年
- 抗原来源:
Rabbit
- 目录编号:
TF6924R
- 级别:
I级
- 库存:
10
- 供应商:
晶风生物
- 标记物:
FITC/Alexa/CY357/BIo/HRP
- 克隆性:
Polyclonal
- 保存条件:
-20
- 形态:
Liquid
- 亚型:
IgG
- 免疫原:
KLH conjugated synthetic peptide derived
- 规格:
50ul/100ul/200ul
产品规格:100ul/200ul(部分有50ul,如需更大包装或其他具体规格,请咨询客服)
研究领域:肿瘤 细胞生物 免疫学等
抗体来源:Rabbit
克隆类型:Polyclonal
交叉反应:Human,Mouse,Rat,Dog,Pig,Cow,Rabbit,Sheep,
产品应用:WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 Flow-Cyt=1μg/Test IF=1:100-500 (石蜡切片需做抗原修复)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
性 状:Liquid
浓 度:1mg/ml
免 疫 原:KLH conjugated synthetic peptide derived
亚 型:IgG
纯化方法:affinity purified by Protein A
储 存 液:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件:Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
CCDC90A卷曲螺旋结构域蛋白90A抗体相关抗体示例(非本抗体,如需本抗体,请联系客服索要说明书):
Sample:
Liver (Mouse) Lysate at 40 ug
Spleen (Mouse) Lysate at 40 ug
NIH/3T3 (Mouse) CellLysate at 30 ug
RAW246.7 (Mouse) CellLysate at 30 ug
Primary: Anti- IL12 at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 22 kD
Observed band size: 35/36 kD
paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (IL12) Polyclonal Antibody, Unconjugated at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) instructions and DAB staining.

paraffin embedded (rat brain tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (IL12) Polyclonal Antibody, Unconjugated at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) instructionsand DAB staining.

CCDC90A卷曲螺旋结构域蛋白90A抗体
Blank control (blue line): Mouse spleen (blue).
Primary Antibody (green line): Rabbit Anti- IL12 antibody
Dilution: 1μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody (white blue line): Goat anti-rabbit IgG-FITC
Dilution: 1μg /test.
Protocol
The cells were fixed with 70% ice-cold methanol overnight at 4℃. Cells stained with Primary Antibody for 30 min at room temperature. The cells were then incubated in 1 X PBS/2%BSA/10% goat serum to block non-specific protein-protein interactions followed by the antibody for 15 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.

Tissue/cell: rat colitis tissue; paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,at 37℃ for 20 min;
Incubation: Anti-IL-12 Polyclonal Antibody, Unconjugated 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB staining
欢迎新老客户咨询订购:CCDC90A卷曲螺旋结构域蛋白90A抗体
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文献和实验PTD介导的蛋白质转导技术及其在神经系统 疾病治疗中的应用 生理状态下,真核生物细胞膜仅对少量小分子具有通透性,大大限制了生物大分子药物对疾病的治疗。由于转基因技术可操作性差、转运效率低、具有潜在的生物毒性作用、并且并非所有细胞均能被转染等缺点,限制了其实际应用[1]。PTD介导的蛋白质转导技术则使大分子蛋白质或多肽自由通透细胞膜成为可能,随之产生疾病蛋白质治疗技术。本文就这一技术及其在神经系统疾病治疗中的意义作一综述。 1.蛋白质转导域(protein transduction
的;但在体外(in vitro),M1-RNA可以独自发挥催化的功能。[1]C5蛋白的作用主要是增强底物结合的亲和力,而活性位点金属离子结合亲和力的提高很可能能够加强M1-RNA的催化速率。细菌核糖核酸酶P的晶体结构显示它具有一个由共轴堆积的螺旋结构域构成的平整表面,这样一个平的表面有助于底物前体tRNA分子的结合和剪切。[5] 古菌中 在古菌中,核糖核酸酶 P含有4-5个结合RNA的蛋白质亚基。体外重构实验显示,这些亚基中的每一个都通过核糖核酸酶P中的RNA组分参与
以及可用于结合其它调控蛋白的调节域。 2)结构特征基本有三大类,即螺旋/转折/螺旋 (H/T/H);锌指 (Zinc finger) 结构以及通常包括亮氨酸拉链(Leu elne zipper)在内的螺旋/环/螺旋 (H/L/H )结构。 3)转录调控的结构域内主要可有带负电荷的螺旋结构{富含谷氨酰胺或寓含腑氨酸的结构,以及其它不规则的含有双性a螺旋 及其外的酸性氨基酸残基等。 4)同(异)源二聚体形成的结构基础:在调控元件中的回文结构及串联序列的存在表明反式因子二聚化可能是蛋白质与DNA
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