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- 详细信息
- 文献和实验
- 技术资料
- 抗体名:
神经内分泌肽QRFP抗体
- 抗体英文名:
QRFP
- 靶点:
细胞浆 细胞膜 分泌型蛋白
- 浓度:
1mg/ml
- 应用范围:
Elisa=1:500-1000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500,ICC=1:100-500,
- 宿主:
Rabbit
- 适应物种:
Human,Mouse,Rat,Dog,Pig,Cow,Rabbit,Sheep,Guinea Pig,
- 保质期:
一年
- 抗原来源:
Rabbit
- 目录编号:
TF11534R
- 级别:
I级
- 库存:
10
- 供应商:
晶风生物
- 标记物:
FITC/Alexa/CY357/BIo/HRP
- 克隆性:
Polyclonal
- 保存条件:
-20
- 形态:
Liquid
- 亚型:
IgG
- 免疫原:
KLH conjugated synthetic peptide derived
- 规格:
50ul/100ul/200ul
产品规格:100ul/200ul(部分有50ul,如需更大包装或其他具体规格,请咨询客服)
研究领域:肿瘤 细胞生物 免疫学等
抗体来源:Rabbit
克隆类型:Polyclonal
交叉反应:Human,Mouse,Rat,Dog,Pig,Cow,Rabbit,Sheep,
产品应用:WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 Flow-Cyt=1μg/Test IF=1:100-500 (石蜡切片需做抗原修复)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
性 状:Liquid
浓 度:1mg/ml
免 疫 原:KLH conjugated synthetic peptide derived
亚 型:IgG
纯化方法:affinity purified by Protein A
储 存 液:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件:Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
QRFP神经内分泌肽QRFP抗体相关抗体示例(非本抗体,如需本抗体,请联系客服索要说明书):
Sample:
Liver (Mouse) Lysate at 40 ug
Spleen (Mouse) Lysate at 40 ug
NIH/3T3 (Mouse) CellLysate at 30 ug
RAW246.7 (Mouse) CellLysate at 30 ug
Primary: Anti- IL12 at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 22 kD
Observed band size: 35/36 kD
paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (IL12) Polyclonal Antibody, Unconjugated at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) instructions and DAB staining.

paraffin embedded (rat brain tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (IL12) Polyclonal Antibody, Unconjugated at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) instructionsand DAB staining.

QRFP神经内分泌肽QRFP抗体
Blank control (blue line): Mouse spleen (blue).
Primary Antibody (green line): Rabbit Anti- IL12 antibody
Dilution: 1μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody (white blue line): Goat anti-rabbit IgG-FITC
Dilution: 1μg /test.
Protocol
The cells were fixed with 70% ice-cold methanol overnight at 4℃. Cells stained with Primary Antibody for 30 min at room temperature. The cells were then incubated in 1 X PBS/2%BSA/10% goat serum to block non-specific protein-protein interactions followed by the antibody for 15 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.

Tissue/cell: rat colitis tissue; paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,at 37℃ for 20 min;
Incubation: Anti-IL-12 Polyclonal Antibody, Unconjugated 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB staining
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文献和实验细胞产生的神经内分泌肽 名称 产生细胞 名称 产生细胞 ACTH 淋巴细胞和巨噬细胞性 生长抑制素 单核细胞、肥大细胞、多形核白细胞 内啡肽 淋巴细胞、巨噬细胞 脑啡肽 辅助性T细胞 生长激素 淋巴细胞 精氨酸升压素(AVP) 胸腺上皮细胞 生乳素 淋巴细胞 催产素 胸腺上皮细胞 绒毛膜促性腺素 T细胞 神经垂体激素运载蛋白 血管活性肠肽(VIP) 单核细胞、肥大细胞、多形核白细胞 TSH 胸腺上皮细胞、T细胞 (二)免疫细胞产生的细胞因子对神经内分泌系统的作用 免疫
免疫系统可以通过多种途径影响神经内分泌功能。免疫细胞本身可以产生和释放内分泌激素,也可以通过它们所产生的细胞因子作用于神经内分泌及全身各器官系统。 (一)免疫细胞产生的内分泌激素 1980年Blalocd等证明人白细胞干扰素中有ACTH和r-内啡肽的活性片段。他们用胃蛋白酶消化人白细胞干扰素,可得到具有ACTH活性的小分子片段。经受体分析也证明,人IFN-α能与与3H双氢吗啡竞争受体,且比吗啡强300倍以上。他们用抗ACTH和r-内啡肽的荧光抗体染色,发现
观察到体内一些分泌肽类物质的细胞具有共的细胞化学特性,即具有摄取胺前体、脱去其羧基变为活性胺的能力(amine precursior uptake and decar-boxylation)而命名为APUD细胞,把这些细胞归属为APUD系统。现在,这上概念已扩展为体内分泌肽类和/或胺类活性物质的内分泌细胞的总称,所分泌的活性物质作为激素、神经递质而发挥作用;已知APUD系统的细胞有40多种,已确定的肽类产物有30多种。近年的研究表明,APUD细胞与下丘脑的一些神经内分泌细胞很相似,因此,提出弥散
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