- ￥1880
- 晶风生物
- TF1385R
- 中国
- 2025年07月03日
- Elisa=1:500-1000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500,ICC=1:100-500,
- Rabbit
- Human,Mouse,Rat,Dog,Pig,Cow,Rabbit,Sheep,Guinea Pig,
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- 详细信息
- 文献和实验
- 技术资料
- 抗体名：
整合素样金属蛋白酶与凝血酶1型-12抗体
- 抗体英文名：
ADAMTS12
- 靶点：
细胞浆 细胞膜 分泌型蛋白
- 浓度：
1mg/ml
- 应用范围：
Elisa=1:500-1000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500,ICC=1:100-500,
- 宿主：
Rabbit
- 适应物种：
Human,Mouse,Rat,Dog,Pig,Cow,Rabbit,Sheep,Guinea Pig,
- 保质期：
一年
- 抗原来源：
Rabbit
- 目录编号：
TF1385R
- 级别：
I级
- 库存：
10
- 供应商：
晶风生物
- 标记物：
FITC/Alexa/CY357/BIo/HRP
- 克隆性：
Polyclonal
- 保存条件：
-20
- 形态：
Liquid
- 亚型：
IgG
- 免疫原：
KLH conjugated synthetic peptide derived
- 规格：
50ul/100ul/200ul
产品规格：100ul/200ul(部分有50ul，如需更大包装或其他具体规格，请咨询客服)
研究领域：肿瘤 细胞生物 免疫学等
抗体来源：Rabbit
克隆类型：Polyclonal
交叉反应：Human,Mouse,Rat,Dog,Pig,Cow,Rabbit,Sheep,
产品应用：WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 Flow-Cyt=1μg/Test IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
性 状：Liquid
浓 度：1mg/ml
免 疫 原：KLH conjugated synthetic peptide derived
亚 型：IgG
纯化方法：affinity purified by Protein A
储 存 液：0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件：Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
ADAMTS12整合素样金属蛋白酶与凝血酶1型-12抗体相关抗体示例（非本抗体，如需本抗体，请联系客服索要说明书）：
Sample:
Liver (Mouse) Lysate at 40 ug
Spleen (Mouse) Lysate at 40 ug
NIH/3T3 (Mouse) CellLysate at 30 ug
RAW246.7 (Mouse) CellLysate at 30 ug
Primary: Anti- IL12 at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 22 kD
Observed band size: 35/36 kD
paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (IL12) Polyclonal Antibody, Unconjugated at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) instructions and DAB staining.
paraffin embedded (rat brain tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (IL12) Polyclonal Antibody, Unconjugated at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) instructionsand DAB staining.
ADAMTS12整合素样金属蛋白酶与凝血酶1型-12抗体
Blank control (blue line): Mouse spleen (blue).
Primary Antibody (green line): Rabbit Anti- IL12 antibody
Dilution: 1μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody (white blue line): Goat anti-rabbit IgG-FITC
Dilution: 1μg /test.
Protocol
The cells were fixed with 70% ice-cold methanol overnight at 4℃. Cells stained with Primary Antibody for 30 min at room temperature. The cells were then incubated in 1 X PBS/2%BSA/10% goat serum to block non-specific protein-protein interactions followed by the antibody for 15 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
Tissue/cell: rat colitis tissue; paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,at 37℃ for 20 min;
Incubation: Anti-IL-12 Polyclonal Antibody, Unconjugated 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB staining
欢迎新老客户咨询订购：ADAMTS12整合素样金属蛋白酶与凝血酶1型-12抗体
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文献和实验三句话读懂一篇 CNS：辐射对精子 DNA 的破坏无法修复，还会遗传给后代！| 每天 2 杯咖啡，这类人群的死亡风险翻倍
for ciliary transport。 该研究利用冷冻电镜单颗粒技术解析了近原子分辨率的 IFT-A 复合物结构，进一步分析了其具有致病性的 IFT-A 突变在复合物上的位置，提出了 IFT-A 如何在单体、聚合物和膜结合形式之间的状态转换。 图 9：来源 Cell 10. Circulation：抗 ADAMTS-7 肽疫苗改善动脉粥样硬化和损伤后新生内膜增生 金属蛋白酶 ADAMTS-7 是一种与人类冠状动脉粥样硬化相关的新位点。 2022 年 12 月 23 日，北京大学孔炜、付毅及郑金刚共同
nm 2，MCV,MCH,MCHC数值记忆，判断贫血类型：正细胞贫血 3，MCV和RDW，判断：小细胞不均一性 4，AA，网织红＜0.5% 5，RPI公式 6，网织红发育阶段分型：4型，B型题 7，点彩红细胞增高：金属中毒 8，运动，白细胞增高，边缘池与循环池的平衡 9，稀释液：乙酸 10，白细胞计数不分性别 11，血涂片上10—12，WBC 13—18×10 9 12，白细胞早晨低，下午高 13，中性粒增多： 14，EOS：白天低，夜间高
mM Tris-HCl,2mM ATP,10mM MgSO4,pH7.4中37°C温浴10分钟而破坏.或者把有标签 蛋白通过ATP-agarose或相似的纯化介质或进行离子交换层 析来除去. 标签蛋白被蛋白酶部分降解 加入蛋白酶抑制剂.多条条带可能是由于目的蛋白被蛋白 酶部分降解的结果.在裂解溶液中加入1mM PMSF可能会 使结果变好.一种无毒的水溶性的PMSF替代物是AEBSF, Roche Biochemicals的商品名为Pefabloc SC.注:丝氨酸 蛋白酶抑制剂必须在使用凝血酶或凝血因子Xa
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