- ￥1880
- 晶风生物
- TF2253R
- 中国
- 2025年07月05日
- Elisa=1:500-1000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500,ICC=1:100-500,
- Rabbit
- Human,Mouse,Rat,Dog,Pig,Cow,Rabbit,Sheep,Guinea Pig,
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- 详细信息
- 文献和实验
- 技术资料
- 抗体名：
剪切和多聚腺苷酸化特异性因子4抗体
- 抗体英文名：
CPSF4
- 靶点：
细胞浆 细胞膜 分泌型蛋白
- 浓度：
1mg/ml
- 应用范围：
Elisa=1:500-1000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500,ICC=1:100-500,
- 宿主：
Rabbit
- 适应物种：
Human,Mouse,Rat,Dog,Pig,Cow,Rabbit,Sheep,Guinea Pig,
- 保质期：
一年
- 抗原来源：
Rabbit
- 目录编号：
TF2253R
- 级别：
I级
- 库存：
10
- 供应商：
晶风生物
- 标记物：
FITC/Alexa/CY357/BIo/HRP
- 克隆性：
Polyclonal
- 保存条件：
-20
- 形态：
Liquid
- 亚型：
IgG
- 免疫原：
KLH conjugated synthetic peptide derived
- 规格：
50ul/100ul/200ul
产品规格：100ul/200ul(部分有50ul，如需更大包装或其他具体规格，请咨询客服)
研究领域：肿瘤 细胞生物 免疫学等
抗体来源：Rabbit
克隆类型：Polyclonal
交叉反应：Human,Mouse,Rat,Dog,Pig,Cow,Rabbit,Sheep,
产品应用：WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 Flow-Cyt=1μg/Test IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
性 状：Liquid
浓 度：1mg/ml
免 疫 原：KLH conjugated synthetic peptide derived
亚 型：IgG
纯化方法：affinity purified by Protein A
储 存 液：0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件：Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
CPSF4剪切和多聚腺苷酸化特异性因子4抗体相关抗体示例（非本抗体，如需本抗体，请联系客服索要说明书）：
Sample:
Liver (Mouse) Lysate at 40 ug
Spleen (Mouse) Lysate at 40 ug
NIH/3T3 (Mouse) CellLysate at 30 ug
RAW246.7 (Mouse) CellLysate at 30 ug
Primary: Anti- IL12 at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 22 kD
Observed band size: 35/36 kD
paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (IL12) Polyclonal Antibody, Unconjugated at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) instructions and DAB staining.
paraffin embedded (rat brain tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (IL12) Polyclonal Antibody, Unconjugated at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) instructionsand DAB staining.
CPSF4剪切和多聚腺苷酸化特异性因子4抗体
Blank control (blue line): Mouse spleen (blue).
Primary Antibody (green line): Rabbit Anti- IL12 antibody
Dilution: 1μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody (white blue line): Goat anti-rabbit IgG-FITC
Dilution: 1μg /test.
Protocol
The cells were fixed with 70% ice-cold methanol overnight at 4℃. Cells stained with Primary Antibody for 30 min at room temperature. The cells were then incubated in 1 X PBS/2%BSA/10% goat serum to block non-specific protein-protein interactions followed by the antibody for 15 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
Tissue/cell: rat colitis tissue; paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,at 37℃ for 20 min;
Incubation: Anti-IL-12 Polyclonal Antibody, Unconjugated 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB staining
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文献和实验Science：颉伟 / 陈子江 / 赵涵团队揭示人类早期胚胎翻译组图谱并发现 TPRXs 参与人类合子基因组激活
水平动态变化趋势，而另一半呈现不同的翻译水平动态变化趋势，例如编码表观遗传重编程因子、小 RNA 生物学合成酶等基因。并且这种物种特异性的翻译变化是部分由于（3' UTR）的关键调控序列，例如胞质多聚腺苷酸化元件 (cytoplasmic polyadenylation element, CPE) 和多聚腺苷酸化信号位点 (polyadenylation signal site, PAS) 的差异排布导致的。 人类早期胚胎翻译组数据还显示出一组 Homeobox 转录因子在人类合子基因组激活阶段附近呈现高翻译
。 特点 1. 长度在200-100,000nt 2. 没有编码蛋白质潜能 3. 具有细胞或组织类型特异性 4. 表达量和保守性比mRNA低 5. 部分lncRNA不含有polyA尾巴 6. 部分也会翻译小肽段 • LncRNA功能及作用形式 功能 1. 结合转录因子，干扰其靶基因，从而调控转录 2. 作为分子海绵，吸附miRNA 3. 与调节蛋白结合，影响蛋白多聚物的形成 4. 招募染色质修饰因子，改变染色
（图源：Nature 官网） 阻断细胞因子风暴 细胞因子风暴（cytokine storm）是指大量促炎症细胞因子急剧升高引发过度免疫反应。细胞因子风暴的发生与病毒感染重症化和致死有密切关联。新冠病毒感染重症患者出现了 IL-6，TNF-a，IFN-γ 等促炎性细胞因子显著升高等细胞因子风暴特征。因此，阻断或平息细胞因子风暴成为治疗的策略之一。 细胞因子风暴的发生与 病毒感染重症化和致死有密切关联 (图源：The Lancet 官网)
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