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- 详细信息
- 文献和实验
- 技术资料
- 抗体名:
新朊蛋白抗体
- 抗体英文名:
SPRN
- 靶点:
细胞浆 细胞膜 分泌型蛋白
- 浓度:
1mg/ml
- 应用范围:
Elisa=1:500-1000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500,ICC=1:100-500,
- 宿主:
Rabbit
- 适应物种:
Human,Mouse,Rat,Dog,Pig,Cow,Rabbit,Sheep,Guinea Pig,
- 保质期:
一年
- 抗原来源:
Rabbit
- 目录编号:
TF2149R
- 级别:
I级
- 库存:
10
- 供应商:
晶风生物
- 标记物:
FITC/Alexa/CY357/BIo/HRP
- 克隆性:
Polyclonal
- 保存条件:
-20
- 形态:
Liquid
- 亚型:
IgG
- 免疫原:
KLH conjugated synthetic peptide derived
- 规格:
50ul/100ul/200ul
产品规格:100ul/200ul(部分有50ul,如需更大包装或其他具体规格,请咨询客服)
研究领域:肿瘤 细胞生物 免疫学等
抗体来源:Rabbit
克隆类型:Polyclonal
交叉反应:Human,Mouse,Rat,Dog,Pig,Cow,Rabbit,Sheep,
产品应用:WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 Flow-Cyt=1μg/Test IF=1:100-500 (石蜡切片需做抗原修复)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
性 状:Liquid
浓 度:1mg/ml
免 疫 原:KLH conjugated synthetic peptide derived
亚 型:IgG
纯化方法:affinity purified by Protein A
储 存 液:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件:Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
SPRN新朊蛋白抗体相关抗体示例(非本抗体,如需本抗体,请联系客服索要说明书):
Sample:
Liver (Mouse) Lysate at 40 ug
Spleen (Mouse) Lysate at 40 ug
NIH/3T3 (Mouse) CellLysate at 30 ug
RAW246.7 (Mouse) CellLysate at 30 ug
Primary: Anti- IL12 at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 22 kD
Observed band size: 35/36 kD
paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (IL12) Polyclonal Antibody, Unconjugated at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) instructions and DAB staining.

paraffin embedded (rat brain tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (IL12) Polyclonal Antibody, Unconjugated at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) instructionsand DAB staining.

SPRN新朊蛋白抗体
Blank control (blue line): Mouse spleen (blue).
Primary Antibody (green line): Rabbit Anti- IL12 antibody
Dilution: 1μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody (white blue line): Goat anti-rabbit IgG-FITC
Dilution: 1μg /test.
Protocol
The cells were fixed with 70% ice-cold methanol overnight at 4℃. Cells stained with Primary Antibody for 30 min at room temperature. The cells were then incubated in 1 X PBS/2%BSA/10% goat serum to block non-specific protein-protein interactions followed by the antibody for 15 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.

Tissue/cell: rat colitis tissue; paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,at 37℃ for 20 min;
Incubation: Anti-IL-12 Polyclonal Antibody, Unconjugated 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB staining
欢迎新老客户咨询订购:SPRN新朊蛋白抗体
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文献和实验存在于正常人血清中,但并不引起疾病。该种抗体可能对于清除损伤了的表皮细胞有一定的作用,银屑病中角朊细胞的过度增生可能与这种抗体的沉积有关。与抗角质层自身抗体有关的疾病尚有类风湿关节 炎、关节 病型银屑病、外伤等。 制备的抗角蛋白抗体:近10年来,应用免疫动物的方法制备了多种抗角蛋白多抗或单抗,可以针对不同分子量的角蛋白成分。角蛋白是上皮细胞的中间丝成份,抗角蛋白抗体可以作为上皮组织和由上皮组织起源的肿瘤的有用标记之一。 二、对天疱疮的研究 天疱疮是一类
:正常型蛋白 PrP SC :致病型蛋白 图 3.18 朊蛋白的构型转换 朊病毒的发现在生物学界引起震惊,因为它与目前公认的“中心法则”即生物遗传信息流的方向是“ DNA ? RNA →蛋白质”的传统观念相抵触。 Pursiner 等人阐明羊瘙痒病的发病机制是由于朊病毒分子构象的改变而致病。这一发现开辟了病因学的一个新领域,可能对其他传染性海绵状脑病的发病原理和病因性质,提供一条新的思路,对生物科学的发展具有重大
「生于忧患,死于安乐」,连细胞也是如此?剑桥大学研究揭示「压力」对细胞有何好处…
于分解胞质聚集物,包括阿尔茨海默症的淀粉样蛋白,可阻止蛋白从自然折叠状态朝聚集的朊病毒样转变。 本研究通过体外模拟 BIP-HSP70 底物动力学,发现蛋白在温和的加热条件下保持着聚集状态,随着时间推移与温度的升高,热休克蛋白在 ATP 的作用下,可以催化蛋白质聚集物的溶解,促使聚集物发生解离,从大分子蛋白向小分子蛋白转变,即有助于解开实验中预先形成的蛋白质聚集物。 图 3:来源 Nature Communications 三、开启细胞「压力」的开关,帮助抵御阿尔兹海默症 本文的通讯
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