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- 详细信息
- 文献和实验
- 技术资料
- 抗体名:
甲型流感病毒M2抗体
- 抗体英文名:
H1N1 matrix protein 2
- 靶点:
细胞浆 细胞膜 分泌型蛋白
- 浓度:
1mg/ml
- 应用范围:
Elisa=1:500-1000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500,ICC=1:100-500,
- 宿主:
Rabbit
- 适应物种:
Human,Mouse,Rat,Dog,Pig,Cow,Rabbit,Sheep,Guinea Pig,
- 保质期:
一年
- 抗原来源:
Rabbit
- 目录编号:
TF1430R
- 级别:
I级
- 库存:
10
- 供应商:
晶风生物
- 标记物:
FITC/Alexa/CY357/BIo/HRP
- 克隆性:
Polyclonal
- 保存条件:
-20
- 形态:
Liquid
- 亚型:
IgG
- 免疫原:
KLH conjugated synthetic peptide derived
- 规格:
50ul/100ul/200ul
产品规格:100ul/200ul(部分有50ul,如需更大包装或其他具体规格,请咨询客服)
研究领域:肿瘤 细胞生物 免疫学等
抗体来源:Rabbit
克隆类型:Polyclonal
交叉反应:Human,Mouse,Rat,Dog,Pig,Cow,Rabbit,Sheep,
产品应用:WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 Flow-Cyt=1μg/Test IF=1:100-500 (石蜡切片需做抗原修复)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
性 状:Liquid
浓 度:1mg/ml
免 疫 原:KLH conjugated synthetic peptide derived
亚 型:IgG
纯化方法:affinity purified by Protein A
储 存 液:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件:Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
H1N1matrixprotein2甲型流感病毒M2抗体相关抗体示例(非本抗体,如需本抗体,请联系客服索要说明书):
Sample:
Liver (Mouse) Lysate at 40 ug
Spleen (Mouse) Lysate at 40 ug
NIH/3T3 (Mouse) CellLysate at 30 ug
RAW246.7 (Mouse) CellLysate at 30 ug
Primary: Anti- IL12 at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 22 kD
Observed band size: 35/36 kD
paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (IL12) Polyclonal Antibody, Unconjugated at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) instructions and DAB staining.

paraffin embedded (rat brain tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (IL12) Polyclonal Antibody, Unconjugated at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) instructionsand DAB staining.

H1N1matrixprotein2甲型流感病毒M2抗体
Blank control (blue line): Mouse spleen (blue).
Primary Antibody (green line): Rabbit Anti- IL12 antibody
Dilution: 1μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody (white blue line): Goat anti-rabbit IgG-FITC
Dilution: 1μg /test.
Protocol
The cells were fixed with 70% ice-cold methanol overnight at 4℃. Cells stained with Primary Antibody for 30 min at room temperature. The cells were then incubated in 1 X PBS/2%BSA/10% goat serum to block non-specific protein-protein interactions followed by the antibody for 15 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.

Tissue/cell: rat colitis tissue; paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,at 37℃ for 20 min;
Incubation: Anti-IL-12 Polyclonal Antibody, Unconjugated 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB staining
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文献和实验余种。值得注意的是:该公司2009年6月先后通过甲型H1N1流感病毒RNA检测试剂盒(荧光PCR法)的质量管理体系考核、取得甲型H1N1流感病毒RNA检测试剂盒(荧光PCR法)的注册受理通知书。2009年6月17日国家食品药品监督管理局医疗器械监管司王宝亭司长、王兰明专员光临我公司并对甲型H1N1流感病毒检测试剂盒文号申报工作进行指导。2009年6月首批428套甲型H1N1流感病毒检测试剂盒配发至全国213个流感检测网络实验室PS:今年甲型流感这一试剂的开发和应用,让金豪公司赚得盆满钵满
重要的情况下,较早的病毒检测方法可能仍然有用。 在过去 20 年中,随着基因组扩增技术的发展,呼吸道病毒检测的敏感性和特异性得到了提高。目前已经发现了多种新的呼吸道病毒,而敏感的 PCR 方法在诊断流程中得以保留。技术的进步促使了多重PCR 检测的开发,该方法在一份 PCR 混合物中使用多个引物,其完成时间较多次单靶点PCR 短。 单一和多重 PCR 可以快速检测临床样本中的呼吸道病毒,并且用于确定新出现的病毒(如甲型流感 H1N1pdm
均后加入细胞培养瓶中,置37℃CO2孵箱培养。(3)MDCK细胞对流感病毒敏感性测试前面提到MDCK细胞对病毒的敏感性随其代数增加而下降。故MDCK细胞在实验室传20代以上时,一般需测试一下其对流感病毒的敏感性。其具体测试方法:选一株对MDCK细胞较敏感的流感病毒株,最好为当前在人群中流行的甲型流感病毒。在同一条件下,用早代与要测试的MDCK细胞对其进行TCID50测定。如果测试的TCID50比早代的低2个或以上Lg,表明其对病毒的敏感性已下降,不应继续使用;如果两者相差不超出一个Lg,表明
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