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- 详细信息
- 文献和实验
- 技术资料
- 抗体名:
单链DNA结合蛋白相互作用蛋白1
- 抗体英文名:
SOSSC
- 靶点:
细胞浆 细胞膜 分泌型蛋白
- 浓度:
1mg/ml
- 应用范围:
Elisa=1:500-1000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500,ICC=1:100-500,
- 宿主:
Rabbit
- 适应物种:
Human,Mouse,Rat,Dog,Pig,Cow,Rabbit,Sheep,Guinea Pig,
- 保质期:
一年
- 抗原来源:
Rabbit
- 目录编号:
TF9491R
- 级别:
I级
- 库存:
10
- 供应商:
晶风生物
- 标记物:
FITC/Alexa/CY357/BIo/HRP
- 克隆性:
Polyclonal
- 保存条件:
-20
- 形态:
Liquid
- 亚型:
IgG
- 免疫原:
KLH conjugated synthetic peptide derived
- 规格:
50ul/100ul/200ul
产品规格:100ul/200ul(部分有50ul,如需更大包装或其他具体规格,请咨询客服)
研究领域:肿瘤 细胞生物 免疫学等
抗体来源:Rabbit
克隆类型:Polyclonal
交叉反应:Human,Mouse,Rat,Dog,Pig,Cow,Rabbit,Sheep,
产品应用:WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 Flow-Cyt=1μg/Test IF=1:100-500 (石蜡切片需做抗原修复)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
性 状:Liquid
浓 度:1mg/ml
免 疫 原:KLH conjugated synthetic peptide derived
亚 型:IgG
纯化方法:affinity purified by Protein A
储 存 液:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件:Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
SOSSC单链DNA结合蛋白相互作用蛋白1相关抗体示例(非本抗体,如需本抗体,请联系客服索要说明书):
Sample:
Liver (Mouse) Lysate at 40 ug
Spleen (Mouse) Lysate at 40 ug
NIH/3T3 (Mouse) CellLysate at 30 ug
RAW246.7 (Mouse) CellLysate at 30 ug
Primary: Anti- IL12 at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 22 kD
Observed band size: 35/36 kD
paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (IL12) Polyclonal Antibody, Unconjugated at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) instructions and DAB staining.

paraffin embedded (rat brain tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (IL12) Polyclonal Antibody, Unconjugated at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) instructionsand DAB staining.

SOSSC单链DNA结合蛋白相互作用蛋白1
Blank control (blue line): Mouse spleen (blue).
Primary Antibody (green line): Rabbit Anti- IL12 antibody
Dilution: 1μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody (white blue line): Goat anti-rabbit IgG-FITC
Dilution: 1μg /test.
Protocol
The cells were fixed with 70% ice-cold methanol overnight at 4℃. Cells stained with Primary Antibody for 30 min at room temperature. The cells were then incubated in 1 X PBS/2%BSA/10% goat serum to block non-specific protein-protein interactions followed by the antibody for 15 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.

Tissue/cell: rat colitis tissue; paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,at 37℃ for 20 min;
Incubation: Anti-IL-12 Polyclonal Antibody, Unconjugated 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB staining
欢迎新老客户咨询订购:SOSSC单链DNA结合蛋白相互作用蛋白1
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文献和实验一、引言 在许多的细胞生命活动中,例如 DNA 复制、mRNA 转录与修饰以及病毒的感染等都涉及到 DNA 与蛋白质之间的相互作用的问题。 重组 DNA 技术的发展,人们已分离到了许多重要的基因。现在的关键问题是需要揭示环境因子及发育信号究竟是如何控制基因的转录活性。为此需要: 1. 鉴定分析参与基因表达调控的 DNA 元件。 2. 分离并鉴定这些顺式元件特异性结合的蛋白质因子。 这些问题的研究都涉及到 DNA 与蛋白质之间的相互作用。 研究 DNA-蛋白质相互作用的实验方法主要包括
单链DNA结合蛋白质 single stranded DNAbinding protein
为DNA蛋白质之一种,是与单链 DNA有强的亲和性,与 DNA复制、遗传的重组等 DNA代谢有关的蛋白质。也称螺旋不稳定蛋白质( helix destabili- zing protein),又称解 DNA旋卷蛋白质( DNAuntwisting protein)。此蛋白质与单链 DNA协同( cooperative)结合,其结合与 DNA碱基的排列是非特异性的。此蛋白质结合的 DNA使 DNA多聚酶的活性增高,并促进重组过程。单链 DNA结合蛋白质在生物界广泛存在, B.
单链结合蛋白 single strand binding protein
这种蛋白又称为双螺旋稳定蛋白(helix destabilizing protein)。当解旋酶将双链打开以后,单链DNA具有一种潜在的恢复原来双链的能力,重新形成氢键。而且单链本身若有反向重复也会形成发夹结构,这两种情况都会影响复制的,但SSB可以解决这一问题。SSB并不是酶,是由177个aa组成的蛋白,E.coli 的SSB以四聚存在,分子量为74KDa,结合在单链上,每个分子可以覆盖32Nt,使DNA受到保护,不被酶水解,也不回复成双链,因此可以降低DNA的Tm值。SSB
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