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- 详细信息
- 询价记录
- 文献和实验
- 技术资料
- 抗体名:
人类免疫缺陷病毒2型/2型艾滋病病毒gp36抗体
- 抗体英文名:
HIV2 gp36
- 靶点:
细胞浆 细胞膜 分泌型蛋白
- 浓度:
1mg/ml
- 应用范围:
Elisa=1:500-1000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500,ICC=1:100-500,
- 宿主:
Rabbit
- 适应物种:
Human,Mouse,Rat,Dog,Pig,Cow,Rabbit,Sheep,Guinea Pig,
- 保质期:
一年
- 抗原来源:
Rabbit
- 目录编号:
TF6410R
- 级别:
I级
- 库存:
10
- 供应商:
晶风生物
- 标记物:
FITC/Alexa/CY357/BIo/HRP
- 克隆性:
Polyclonal
- 保存条件:
-20
- 形态:
Liquid
- 亚型:
IgG
- 免疫原:
KLH conjugated synthetic peptide derived
- 规格:
50ul/100ul/200ul
产品规格:100ul/200ul(部分有50ul,如需更大包装或其他具体规格,请咨询客服)
研究领域:肿瘤 细胞生物 免疫学等
抗体来源:Rabbit
克隆类型:Polyclonal
交叉反应:Human,Mouse,Rat,Dog,Pig,Cow,Rabbit,Sheep,
产品应用:WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 Flow-Cyt=1μg/Test IF=1:100-500 (石蜡切片需做抗原修复)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
性 状:Liquid
浓 度:1mg/ml
免 疫 原:KLH conjugated synthetic peptide derived
亚 型:IgG
纯化方法:affinity purified by Protein A
储 存 液:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件:Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
HIV2gp36人类免疫缺陷病毒2型/2型艾滋病病毒gp36抗体相关抗体示例(非本抗体,如需本抗体,请联系客服索要说明书):
Sample:
Liver (Mouse) Lysate at 40 ug
Spleen (Mouse) Lysate at 40 ug
NIH/3T3 (Mouse) CellLysate at 30 ug
RAW246.7 (Mouse) CellLysate at 30 ug
Primary: Anti- IL12 at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 22 kD
Observed band size: 35/36 kD
paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (IL12) Polyclonal Antibody, Unconjugated at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) instructions and DAB staining.

paraffin embedded (rat brain tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (IL12) Polyclonal Antibody, Unconjugated at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) instructionsand DAB staining.

HIV2gp36人类免疫缺陷病毒2型/2型艾滋病病毒gp36抗体
Blank control (blue line): Mouse spleen (blue).
Primary Antibody (green line): Rabbit Anti- IL12 antibody
Dilution: 1μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody (white blue line): Goat anti-rabbit IgG-FITC
Dilution: 1μg /test.
Protocol
The cells were fixed with 70% ice-cold methanol overnight at 4℃. Cells stained with Primary Antibody for 30 min at room temperature. The cells were then incubated in 1 X PBS/2%BSA/10% goat serum to block non-specific protein-protein interactions followed by the antibody for 15 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.

Tissue/cell: rat colitis tissue; paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,at 37℃ for 20 min;
Incubation: Anti-IL-12 Polyclonal Antibody, Unconjugated 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB staining
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文献和实验。与第三代抗HIV-1/2试剂相比,出时间提前了4~9。其优点在于能同时测抗原抗体,降低血源筛查的残余危险度。 (2),快速测(RT)及其它测 随着对HIV感染者和AIDS病人抗逆转录病毒治疗的进展,及对无症状HIV感染者提供自愿咨询测(VCT)的迫切需求,简便,快速的HIV测方法被广泛应用。常用的主要有以下几种: ①,明胶颗粒凝集试(PA):PA是HIV血清抗体测的一种简便方法,是将HIV抗原致敏明胶颗粒作为载体,与待样品作用,混匀后保温(一般为室温)。当待样品含有HIV抗体时
方法的灵敏度不够而使得其检出时间要比RNA晚。从HIV感染到能够检测出HIV抗体这一时期,被称作“窗口期”。在窗口期,能够通过病毒RNA、p24抗原和CD4淋巴细胞水平来确定HIV感染。CD4淋巴细胞水平随着感染的发展而逐渐下降,当其在血液中细胞下降到200个/mL时,就会发生严重的免疫缺陷,患者就被确诊为艾滋病。病毒RNA、p24抗原、HIV抗体和CD4淋巴细胞水平可以用来确 定HIV感染、检测病情发展。 HIV在病毒分类中属逆转录病毒科慢病毒属中的人类免疫缺陷病毒组。迄今
肽,HIV-1的O组可被检测出来,但其敏感性仍有待进一步的观察,因为HIV任何变异所造成的抗原结构细微改变都可能影响其敏感性。同时,由于现有的无论是血清学检测试剂还是核酸检测试剂都是以流行于欧美的B亚型病毒株为基础的,其对非B亚型毒株检测的敏感性有待进一步证实,有研究报道表明,在抗体阳转期间,应用取自B亚型抗原的抗体检测初筛试剂盒可发现在检测非B亚型时敏感性较低。并且,目前所用的几种用于检测核酸的试剂盒,在检测非B亚型HIV-1感染者时,或者不能检测,或者不能正确定量病毒量。对HIV-2型和HIV
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