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- 详细信息
- 文献和实验
- 技术资料
- 抗体名:
载脂蛋白B-mRNA编辑酶复合物3C抗体
- 抗体英文名:
APOBEC3C
- 靶点:
细胞浆 细胞膜 分泌型蛋白
- 浓度:
1mg/ml
- 应用范围:
Elisa=1:500-1000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500,ICC=1:100-500,
- 宿主:
Rabbit
- 适应物种:
Human,Mouse,Rat,Dog,Pig,Cow,Rabbit,Sheep,Guinea Pig,
- 保质期:
一年
- 抗原来源:
Rabbit
- 目录编号:
TF12495R
- 级别:
I级
- 库存:
10
- 供应商:
晶风生物
- 标记物:
FITC/Alexa/CY357/BIo/HRP
- 克隆性:
Polyclonal
- 保存条件:
-20
- 形态:
Liquid
- 亚型:
IgG
- 免疫原:
KLH conjugated synthetic peptide derived
- 规格:
50ul/100ul/200ul
产品规格:100ul/200ul(部分有50ul,如需更大包装或其他具体规格,请咨询客服)
研究领域:肿瘤 细胞生物 免疫学等
抗体来源:Rabbit
克隆类型:Polyclonal
交叉反应:Human,Mouse,Rat,Dog,Pig,Cow,Rabbit,Sheep,
产品应用:WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 Flow-Cyt=1μg/Test IF=1:100-500 (石蜡切片需做抗原修复)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
性 状:Liquid
浓 度:1mg/ml
免 疫 原:KLH conjugated synthetic peptide derived
亚 型:IgG
纯化方法:affinity purified by Protein A
储 存 液:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件:Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
APOBEC3C载脂蛋白B-mRNA编辑酶复合物3C抗体相关抗体示例(非本抗体,如需本抗体,请联系客服索要说明书):
Sample:
Liver (Mouse) Lysate at 40 ug
Spleen (Mouse) Lysate at 40 ug
NIH/3T3 (Mouse) CellLysate at 30 ug
RAW246.7 (Mouse) CellLysate at 30 ug
Primary: Anti- IL12 at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 22 kD
Observed band size: 35/36 kD
paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (IL12) Polyclonal Antibody, Unconjugated at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) instructions and DAB staining.

paraffin embedded (rat brain tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (IL12) Polyclonal Antibody, Unconjugated at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) instructionsand DAB staining.

APOBEC3C载脂蛋白B-mRNA编辑酶复合物3C抗体
Blank control (blue line): Mouse spleen (blue).
Primary Antibody (green line): Rabbit Anti- IL12 antibody
Dilution: 1μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody (white blue line): Goat anti-rabbit IgG-FITC
Dilution: 1μg /test.
Protocol
The cells were fixed with 70% ice-cold methanol overnight at 4℃. Cells stained with Primary Antibody for 30 min at room temperature. The cells were then incubated in 1 X PBS/2%BSA/10% goat serum to block non-specific protein-protein interactions followed by the antibody for 15 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.

Tissue/cell: rat colitis tissue; paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,at 37℃ for 20 min;
Incubation: Anti-IL-12 Polyclonal Antibody, Unconjugated 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB staining
欢迎新老客户咨询订购:APOBEC3C载脂蛋白B-mRNA编辑酶复合物3C抗体
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文献和实验:①抗原-抗体反应,②与嵌合连接分子结合,③PCR 扩增嵌合连接分子中的 DNA(一般为质粒 DNA)。该技术的关键环节是嵌合连接分子的制备。在免疫-PCR 中,嵌合连接分子起着桥梁作用,它有两个结合位点,一个与抗原抗体复合物中的抗体结合,一个与质粒 DNA 结合,其基本原理与 ELISA 和免疫酶染色相似,不同之处在于其中的标记物不是酶而是质粒 DNA,在操作反应中形成抗原抗体-连接分子-DNA 复合物,通过 PCR 扩增 DNA 来判断是否存在特异性抗原。 免疫 PCR 优点为:①特异
of Emotional Information② Nature首次发现:与细胞应激相关的蛋白也会调节细胞分裂肝癌是全世界造成癌症死亡的第三大杀手,更是有中国特色的一种癌症。诱发肝癌的因素很多,包括病毒、环境和遗传因素的共同作用,然而研究多年,至今科学家们还没有找到治愈肝癌的可靠方法。来自西班牙CNIC心血管研究中心的研究人员发现参与细胞应激反应的酶可能成为治疗肝癌的新药理学靶点,他们指出p38激酶四种类型之一:p38γ对肝细胞分裂影响重大,参与了肝细胞分裂的起始步骤。这一研究成果公布在4月10日的Nature杂志
作用于靶mRNA的SD序列和部分编码区,直接抑制翻译,或与靶mRNA结合形成双链RNA,从而易被RNA酶 Ⅲ 降解; Ⅱ类反义RNA与mRNA的非编码区结合,引起mRNA构象变化,抑制翻译; Ⅲ类反义RNA则直接抑制靶mRNA的转录。 双链RNA进入细胞后,能够在Dicer酶的作用下被裂解成siRNA,而另一方面双链RNA还能在特定聚合酶下形成单链,并和某些蛋白形成复合物使mRNA被RNA酶裂解,并且以siRNA作为引物,以mRNA为模板形成双链RNA。最后形成siRNA聚合酶链式
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