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- 详细信息
- 文献和实验
- 技术资料
- 抗体名:
受体结合丝氨酸苏氨酸激酶3抗体
- 抗体英文名:
RIPK3
- 靶点:
细胞浆 细胞膜 分泌型蛋白
- 浓度:
1mg/ml
- 应用范围:
Elisa=1:500-1000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500,ICC=1:100-500,
- 宿主:
Rabbit
- 适应物种:
Human,Mouse,Rat,Dog,Pig,Cow,Rabbit,Sheep,Guinea Pig,
- 保质期:
一年
- 抗原来源:
Rabbit
- 目录编号:
TF3551R
- 级别:
I级
- 库存:
10
- 供应商:
晶风生物
- 标记物:
FITC/Alexa/CY357/BIo/HRP
- 克隆性:
Polyclonal
- 保存条件:
-20
- 形态:
Liquid
- 亚型:
IgG
- 免疫原:
KLH conjugated synthetic peptide derived
- 规格:
50ul/100ul/200ul
产品规格:100ul/200ul(部分有50ul,如需更大包装或其他具体规格,请咨询客服)
研究领域:肿瘤 细胞生物 免疫学等
抗体来源:Rabbit
克隆类型:Polyclonal
交叉反应:Human,Mouse,Rat,Dog,Pig,Cow,Rabbit,Sheep,
产品应用:WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 Flow-Cyt=1μg/Test IF=1:100-500 (石蜡切片需做抗原修复)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
性 状:Liquid
浓 度:1mg/ml
免 疫 原:KLH conjugated synthetic peptide derived
亚 型:IgG
纯化方法:affinity purified by Protein A
储 存 液:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件:Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
RIPK3受体结合丝氨酸苏氨酸激酶3抗体相关抗体示例(非本抗体,如需本抗体,请联系客服索要说明书):
Sample:
Liver (Mouse) Lysate at 40 ug
Spleen (Mouse) Lysate at 40 ug
NIH/3T3 (Mouse) CellLysate at 30 ug
RAW246.7 (Mouse) CellLysate at 30 ug
Primary: Anti- IL12 at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 22 kD
Observed band size: 35/36 kD
paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (IL12) Polyclonal Antibody, Unconjugated at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) instructions and DAB staining.

paraffin embedded (rat brain tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (IL12) Polyclonal Antibody, Unconjugated at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) instructionsand DAB staining.

RIPK3受体结合丝氨酸苏氨酸激酶3抗体
Blank control (blue line): Mouse spleen (blue).
Primary Antibody (green line): Rabbit Anti- IL12 antibody
Dilution: 1μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody (white blue line): Goat anti-rabbit IgG-FITC
Dilution: 1μg /test.
Protocol
The cells were fixed with 70% ice-cold methanol overnight at 4℃. Cells stained with Primary Antibody for 30 min at room temperature. The cells were then incubated in 1 X PBS/2%BSA/10% goat serum to block non-specific protein-protein interactions followed by the antibody for 15 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.

Tissue/cell: rat colitis tissue; paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,at 37℃ for 20 min;
Incubation: Anti-IL-12 Polyclonal Antibody, Unconjugated 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB staining
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文献和实验蛋白丝氨酸/苏氨酸激酶 功能是使蛋白质上的丝氨酸和苏氨酸残基发生磷酸化。除了参与协同刺激信号的启动之外,这类激酶一般在信号转导的中下游发挥作用。由于信号途径具有相互“串流”(cross-talking)的特点,蛋白丝/苏氨酸激酶往往为各种免疫细胞和不同受体介导的信号途径所共用。与免疫细胞活化有关的丝/苏氨酸激酶主要有: 蛋白激酶C(PKC):参与淋巴细胞激活信号转导的磷脂酰肌醇途径,激活转录因子NF-kB;
是一类磷酸转移酶,作用是把 ATP 的磷酸基转移到它底物的 某个蛋白的特定的氨基酸残基上面去,从而就改变了这个下游蛋白的构象。酪氨酸激酶 (PTK) 和丝氨酸 / 苏氨酸激酶 (STK)3、转录因子(transcription factors)对基因转录有调节作用的蛋白,那么细胞对信号转导有诸多 反应,最终都是涉及到蛋白和 DNA 相互识别和相互作用,引起一些基因表达 的改变,所以也有人把信号通路当中的转录因子统称为第 3 信使:第一信使是配体,与受体结合;第二信使是配体和受体结合之后激活的胞内
参与免疫细胞激活信号转导的蛋白磷酸酶 蛋白磷酸酶的作用和蛋白激酶相反。根据脱磷酸化的氨基酸残基的不同,蛋白磷酸酶也分成蛋白酪氨酸磷酸酶(PTP,PTPase)和丝氨酸/苏氨酸磷酸酶。参与淋巴细胞激活的蛋白磷酸酶主要有: ①CD45:该分子胞内段的两个结构域发挥PTP的作用,因而CD45属于受体型蛋白酪氨酸磷酸酶,在对抗瓢kPTK的作用和启动淋巴细胞信号转导中发挥关键作用; ②钙调磷酸
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