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- 详细信息
- 文献和实验
- 技术资料
- 抗体名:
酸性神经鞘磷脂酶抗体
- 抗体英文名:
Acid sphingomyelinase
- 靶点:
细胞浆 细胞膜 分泌型蛋白
- 浓度:
1mg/ml
- 应用范围:
Elisa=1:500-1000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500,ICC=1:100-500,
- 宿主:
Rabbit
- 适应物种:
Human,Mouse,Rat,Dog,Pig,Cow,Rabbit,Sheep,Guinea Pig,
- 保质期:
一年
- 抗原来源:
Rabbit
- 目录编号:
TF6318R
- 级别:
I级
- 库存:
10
- 供应商:
晶风生物
- 标记物:
FITC/Alexa/CY357/BIo/HRP
- 克隆性:
Polyclonal
- 保存条件:
-20
- 形态:
Liquid
- 亚型:
IgG
- 免疫原:
KLH conjugated synthetic peptide derived
- 规格:
50ul/100ul/200ul
产品规格:100ul/200ul(部分有50ul,如需更大包装或其他具体规格,请咨询客服)
研究领域:肿瘤 细胞生物 免疫学等
抗体来源:Rabbit
克隆类型:Polyclonal
交叉反应:Human,Mouse,Rat,Dog,Pig,Cow,Rabbit,Sheep,
产品应用:WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 Flow-Cyt=1μg/Test IF=1:100-500 (石蜡切片需做抗原修复)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
性 状:Liquid
浓 度:1mg/ml
免 疫 原:KLH conjugated synthetic peptide derived
亚 型:IgG
纯化方法:affinity purified by Protein A
储 存 液:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件:Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Acidsphingomyelinase酸性神经鞘磷脂酶抗体相关抗体示例(非本抗体,如需本抗体,请联系客服索要说明书):
Sample:
Liver (Mouse) Lysate at 40 ug
Spleen (Mouse) Lysate at 40 ug
NIH/3T3 (Mouse) CellLysate at 30 ug
RAW246.7 (Mouse) CellLysate at 30 ug
Primary: Anti- IL12 at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 22 kD
Observed band size: 35/36 kD
paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (IL12) Polyclonal Antibody, Unconjugated at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) instructions and DAB staining.

paraffin embedded (rat brain tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (IL12) Polyclonal Antibody, Unconjugated at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) instructionsand DAB staining.

Acidsphingomyelinase酸性神经鞘磷脂酶抗体
Blank control (blue line): Mouse spleen (blue).
Primary Antibody (green line): Rabbit Anti- IL12 antibody
Dilution: 1μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody (white blue line): Goat anti-rabbit IgG-FITC
Dilution: 1μg /test.
Protocol
The cells were fixed with 70% ice-cold methanol overnight at 4℃. Cells stained with Primary Antibody for 30 min at room temperature. The cells were then incubated in 1 X PBS/2%BSA/10% goat serum to block non-specific protein-protein interactions followed by the antibody for 15 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.

Tissue/cell: rat colitis tissue; paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,at 37℃ for 20 min;
Incubation: Anti-IL-12 Polyclonal Antibody, Unconjugated 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB staining
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文献和实验大鼠神经胶质纤维酸性蛋白(GFAP)酶联免疫分析(ELISA)
大鼠神经胶质纤维酸性蛋白 (GFAP) 酶联免疫分析( ELISA ) 试剂盒使用说明书 本试剂仅供研究使用 目的:本试剂盒用于测定大鼠血清,血浆及相关液体样本中 神经胶质纤维酸性蛋白(GFAP) 含量。 实验原理: 本试剂盒应用双抗体夹心法测定标本中大鼠神经胶质纤维酸性蛋白 (GFAP) 水平。用纯化的大鼠神经胶质纤维酸性蛋白 (GFAP) 抗体包被微孔板,制成固相抗体,往包被单抗的微孔中依次加入神经胶质纤维酸性
能减少失败的概率,所以同学们做实验时也不要过于有心里负担,怕这怕那的,要保持一个自然的心态,否则容易抑郁。今天我们就来看一看生物信息学的各种神器是如何来帮助我们的 Western blot 实验。大家在查找抗体的时候有没有看见免疫原的序列和这两个图标呢?这个意思就是说厂家在生产这个抗体的时候,先是合成了一段肽链,这个肽链的氨基酸序列是从蛋白质序列里面摘录的,下面 blast with 意思就是分别和 NCBI 的数据库或 Expasy 的蛋白质数据库里的蛋白质氨基酸序列进行比对。这个 blast
如下: 1.确定细胞类型和形态组织细胞内有些蛋白具有组织特异性,如胶质原纤维酸性蛋白(gial fibrillary acidic protein,GFAP)只存在于星形胶质细胞内.神经丝蛋白(Neurofilament,NF)只存在于神经细胞内。通常把这些具有组织特异性的蛋白称为标记性蛋白(Proteiniliaker)。通过标记性蛋白的特异性抗体可确定细胞种类。有些细胞(如表皮内朗格汉斯细胞和黑色素细胞等)在光镜下不易辨认,通过对胞质内的特定蛋白实施免疫 组化染色,便能清楚显示此类
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