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- 详细信息
- 文献和实验
- 技术资料
- 抗体名:
脊髓灰质炎病毒受体相关蛋白4抗体
- 抗体英文名:
Nectin 4
- 靶点:
细胞浆 细胞膜 分泌型蛋白
- 浓度:
1mg/ml
- 应用范围:
Elisa=1:500-1000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500,ICC=1:100-500,
- 宿主:
Rabbit
- 适应物种:
Human,Mouse,Rat,Dog,Pig,Cow,Rabbit,Sheep,Guinea Pig,
- 保质期:
一年
- 抗原来源:
Rabbit
- 目录编号:
TF10029R
- 级别:
I级
- 库存:
10
- 供应商:
晶风生物
- 标记物:
FITC/Alexa/CY357/BIo/HRP
- 克隆性:
Polyclonal
- 保存条件:
-20
- 形态:
Liquid
- 亚型:
IgG
- 免疫原:
KLH conjugated synthetic peptide derived
- 规格:
50ul/100ul/200ul
产品规格:100ul/200ul(部分有50ul,如需更大包装或其他具体规格,请咨询客服)
研究领域:肿瘤 细胞生物 免疫学等
抗体来源:Rabbit
克隆类型:Polyclonal
交叉反应:Human,Mouse,Rat,Dog,Pig,Cow,Rabbit,Sheep,
产品应用:WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 Flow-Cyt=1μg/Test IF=1:100-500 (石蜡切片需做抗原修复)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
性 状:Liquid
浓 度:1mg/ml
免 疫 原:KLH conjugated synthetic peptide derived
亚 型:IgG
纯化方法:affinity purified by Protein A
储 存 液:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件:Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Nectin4脊髓灰质炎病毒受体相关蛋白4抗体相关抗体示例(非本抗体,如需本抗体,请联系客服索要说明书):
Sample:
Liver (Mouse) Lysate at 40 ug
Spleen (Mouse) Lysate at 40 ug
NIH/3T3 (Mouse) CellLysate at 30 ug
RAW246.7 (Mouse) CellLysate at 30 ug
Primary: Anti- IL12 at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 22 kD
Observed band size: 35/36 kD
paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (IL12) Polyclonal Antibody, Unconjugated at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) instructions and DAB staining.

paraffin embedded (rat brain tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (IL12) Polyclonal Antibody, Unconjugated at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) instructionsand DAB staining.

Nectin4脊髓灰质炎病毒受体相关蛋白4抗体
Blank control (blue line): Mouse spleen (blue).
Primary Antibody (green line): Rabbit Anti- IL12 antibody
Dilution: 1μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody (white blue line): Goat anti-rabbit IgG-FITC
Dilution: 1μg /test.
Protocol
The cells were fixed with 70% ice-cold methanol overnight at 4℃. Cells stained with Primary Antibody for 30 min at room temperature. The cells were then incubated in 1 X PBS/2%BSA/10% goat serum to block non-specific protein-protein interactions followed by the antibody for 15 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.

Tissue/cell: rat colitis tissue; paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,at 37℃ for 20 min;
Incubation: Anti-IL-12 Polyclonal Antibody, Unconjugated 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB staining
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文献和实验可用于传达有关传感器表面的信息,因此 SPR 可用于研究游离分析物分子和溶液之间的分子相互作用,以及与固定在传感器表面上的探针分子的分子相互作用。 ③ SERS 是一种振动光谱技术,已被应用于检测 Aβ 肽和 t-tau 蛋白,灵敏度低至单分子水平。基于 SERS 的独特性质,分析物的化学键信息,如酰胺I带(拉曼峰在∼1650 cm−1处)、组氨酸带(拉曼峰在 ∼1440 cm−1处)和蛋白质-CN 条带(拉曼峰在∼1090 cm−1),从而获得 AD 相关蛋白标志物与相应抗体的特异性结合
性。近交系小鼠接种少量单纯疱疹病毒便可致命,而其他小鼠可耐受较大剂量病毒的攻击不出现症状,涉及的基因与MHC有关。在人体中难以做出评价,但随着免疫应答基因的深入研究有助于阐明这一问题。 许多病毒有限定的宿主范围,种属免疫可能取决于宿主细胞是否具有相应的受体,如脊液灰质炎病毒的受体仅存在于人和灵长类动物中,因而其他动物具有天然的种属免疫力;人类免疫缺陷病毒、肝炎病毒也是如此。相反,狂犬病毒可感染多种温血动物。 二、抗体介导的抗病毒作用 (一)中和抗体理与非中和抗体 具有
暴发流行。引起流行的病毒型别以Ⅰ型居多。潜伏期通常为7~14天,最短2天,最长35天。在临床症状出现前后病人均具有传染性。 三、临床表现 人是脊髓灰质炎病毒的唯一天然宿主,这是因为在人细胞膜表面有一种受体,与病毒衣壳上的结构蛋白VP1具有特异的亲和力,使病毒得以吸附到细胞上。受病毒感染后,绝大多数人(90~95%)呈隐型感染,而显性感染者也多为轻症感染(4~ 8%),只有少数病人(1~2%)发生神经系统感染,引起严重的症状和后果。根据显性感染病人的临床表现可分为三种类型。①轻型
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