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- 详细信息
- 文献和实验
- 技术资料
- 抗体名:
细胞衰老抑制基因抗体
- 抗体英文名:
RSL1D1
- 靶点:
细胞浆 细胞膜 分泌型蛋白
- 浓度:
1mg/ml
- 应用范围:
Elisa=1:500-1000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500,ICC=1:100-500,
- 宿主:
Rabbit
- 适应物种:
Human,Mouse,Rat,Dog,Pig,Cow,Rabbit,Sheep,Guinea Pig,
- 保质期:
一年
- 抗原来源:
Rabbit
- 目录编号:
TF0793R
- 级别:
I级
- 库存:
10
- 供应商:
晶风生物
- 标记物:
FITC/Alexa/CY357/BIo/HRP
- 克隆性:
Polyclonal
- 保存条件:
-20
- 形态:
Liquid
- 亚型:
IgG
- 免疫原:
KLH conjugated synthetic peptide derived
- 规格:
50ul/100ul/200ul
产品规格:100ul/200ul(部分有50ul,如需更大包装或其他具体规格,请咨询客服)
研究领域:肿瘤 细胞生物 免疫学等
抗体来源:Rabbit
克隆类型:Polyclonal
交叉反应:Human,Mouse,Rat,Dog,Pig,Cow,Rabbit,Sheep,
产品应用:WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 Flow-Cyt=1μg/Test IF=1:100-500 (石蜡切片需做抗原修复)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
性 状:Liquid
浓 度:1mg/ml
免 疫 原:KLH conjugated synthetic peptide derived
亚 型:IgG
纯化方法:affinity purified by Protein A
储 存 液:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件:Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
RSL1D1细胞衰老抑制基因抗体相关抗体示例(非本抗体,如需本抗体,请联系客服索要说明书):
Sample:
Liver (Mouse) Lysate at 40 ug
Spleen (Mouse) Lysate at 40 ug
NIH/3T3 (Mouse) CellLysate at 30 ug
RAW246.7 (Mouse) CellLysate at 30 ug
Primary: Anti- IL12 at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 22 kD
Observed band size: 35/36 kD
paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (IL12) Polyclonal Antibody, Unconjugated at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) instructions and DAB staining.

paraffin embedded (rat brain tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (IL12) Polyclonal Antibody, Unconjugated at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) instructionsand DAB staining.

RSL1D1细胞衰老抑制基因抗体
Blank control (blue line): Mouse spleen (blue).
Primary Antibody (green line): Rabbit Anti- IL12 antibody
Dilution: 1μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody (white blue line): Goat anti-rabbit IgG-FITC
Dilution: 1μg /test.
Protocol
The cells were fixed with 70% ice-cold methanol overnight at 4℃. Cells stained with Primary Antibody for 30 min at room temperature. The cells were then incubated in 1 X PBS/2%BSA/10% goat serum to block non-specific protein-protein interactions followed by the antibody for 15 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.

Tissue/cell: rat colitis tissue; paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,at 37℃ for 20 min;
Incubation: Anti-IL-12 Polyclonal Antibody, Unconjugated 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB staining
欢迎新老客户咨询订购:RSL1D1细胞衰老抑制基因抗体
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文献和实验Dev Cell:刘光慧 / 张维绮 / 曲静合作揭示胎盘基因的异常激活是人类细胞衰老的驱动力
基因组图谱,以期探究干细胞在复制性衰老、病理性加速衰老过程中表观基因组的重塑规律。 该研究深度解析了人间充质干细胞衰老过程中核膜和染色质互作的动态变化、染色质在细胞核中径向分布的大规模重排、染色质高级结构的重塑、精细的染色质状态的转变以及不同层次表观基因组信息的相互作用。 研究人员发现衰老细胞的表观基因组呈现出染色质「刚性」的丢失和松弛性增加、表观基因组「熵」和混乱度的增加、「区室化」特征的减弱、全基因组表观信号的趋同性变化和「极性」的降低(激活性基因组区域和抑制性基因组区域表观特征丢失)等规律。研究
缺血再灌注损伤(ischemia/reperfusion Injury,IRI)中,细胞衰老的作用具有双重调节功能,调节衰老细胞可能是改善供体肾脏结果的潜在治疗靶点。人脐带间充质干细胞衍生的外泌体(HUMSC-EVs)在免疫调节和组织微环境稳态中起重要作用,能够通过减少氧化损伤、炎症细胞因子表达、抑制细胞凋亡等机制改善肾脏 IRI 的预后。本文旨在研究 HUMSC-EVs 通过抑制肾小管上皮细胞衰老来实现保护肾脏 IRI 的机制,并探讨了 HUMSC-EVs 与达沙替尼(dasatinib)和槲皮素(quercetin)联合
Cell 重磅 | 刘光慧等揭示靶向古病毒复活或可实现衰老干预
充质干细胞模型,人成纤维细胞衰老模型,以及小鼠、猴和人的生理性及病理性多器官衰老模型),结合高通量链特异性转录本测序、全基因组 DNA 甲基化测序、高分辨率单分子 RNA/DNA 原位杂交、免疫电镜和高灵敏的液滴数字 PCR 等多学科交叉技术,发现衰老细胞中表观遗传去抑制(如异染色质减少)导致基因组中 ERV 的转录激活并翻译出病毒蛋白,进而包装成为病毒颗粒。一方面,衰老细胞中 ERV 的反转录产物通过激活 cGAS-STING 天然免疫通路诱发细胞衰老和炎症;另一方面,衰老细胞释放的 ERV 病毒颗粒可通过旁分泌或体液
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