- ￥1880
- 晶风生物
- TF6697R
- 中国
- 2026年03月16日
- Elisa=1:500-1000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500,ICC=1:100-500,
- Rabbit
- Human,Mouse,Rat,Dog,Pig,Cow,Rabbit,Sheep,Guinea Pig,
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- 详细信息
- 文献和实验
- 技术资料
- 抗体名：
核糖核酸酶Dicer抗体
- 抗体英文名：
DICER1
- 靶点：
细胞浆 细胞膜 分泌型蛋白
- 浓度：
1mg/ml
- 应用范围：
Elisa=1:500-1000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500,ICC=1:100-500,
- 宿主：
Rabbit
- 适应物种：
Human,Mouse,Rat,Dog,Pig,Cow,Rabbit,Sheep,Guinea Pig,
- 保质期：
一年
- 抗原来源：
Rabbit
- 目录编号：
TF6697R
- 级别：
I级
- 库存：
10
- 供应商：
晶风生物
- 标记物：
FITC/Alexa/CY357/BIo/HRP
- 克隆性：
Polyclonal
- 保存条件：
-20
- 形态：
Liquid
- 亚型：
IgG
- 免疫原：
KLH conjugated synthetic peptide derived
- 规格：
50ul/100ul/200ul
产品规格：100ul/200ul(部分有50ul，如需更大包装或其他具体规格，请咨询客服)
研究领域：肿瘤 细胞生物 免疫学等
抗体来源：Rabbit
克隆类型：Polyclonal
交叉反应：Human,Mouse,Rat,Dog,Pig,Cow,Rabbit,Sheep,
产品应用：WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 Flow-Cyt=1μg/Test IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
性 状：Liquid
浓 度：1mg/ml
免 疫 原：KLH conjugated synthetic peptide derived
亚 型：IgG
纯化方法：affinity purified by Protein A
储 存 液：0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件：Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
DICER1核糖核酸酶Dicer抗体相关抗体示例（非本抗体，如需本抗体，请联系客服索要说明书）：
Sample:
Liver (Mouse) Lysate at 40 ug
Spleen (Mouse) Lysate at 40 ug
NIH/3T3 (Mouse) CellLysate at 30 ug
RAW246.7 (Mouse) CellLysate at 30 ug
Primary: Anti- IL12 at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 22 kD
Observed band size: 35/36 kD
paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (IL12) Polyclonal Antibody, Unconjugated at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) instructions and DAB staining.
paraffin embedded (rat brain tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (IL12) Polyclonal Antibody, Unconjugated at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) instructionsand DAB staining.
DICER1核糖核酸酶Dicer抗体
Blank control (blue line): Mouse spleen (blue).
Primary Antibody (green line): Rabbit Anti- IL12 antibody
Dilution: 1μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody (white blue line): Goat anti-rabbit IgG-FITC
Dilution: 1μg /test.
Protocol
The cells were fixed with 70% ice-cold methanol overnight at 4℃. Cells stained with Primary Antibody for 30 min at room temperature. The cells were then incubated in 1 X PBS/2%BSA/10% goat serum to block non-specific protein-protein interactions followed by the antibody for 15 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
Tissue/cell: rat colitis tissue; paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,at 37℃ for 20 min;
Incubation: Anti-IL-12 Polyclonal Antibody, Unconjugated 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB staining
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文献和实验-iTTM RNAi技术允许你利用RNAi的威力――利用各种各样的技术――进行基因阻断的研究。内源RNAi机制RNAi在真核细胞中的发生过程是通过一个被称之为Dicer的酶，将长的dsRNA裂解成21-23核苷酸的短的干扰RNA（siRNA）。siRNA变成细胞内RNA诱导沉默复合体（RNA-induced silencing complex RISC）的一部分，该复合物通过互补靶定将要降解的细胞内的mRNA，最终将细胞中的基因表达阻断。BLOCK-iT™ RNAi技术提供了以下种在细胞中产生siRNA
)。 MiRNA：是内源性的，是一种非编码的RNA;由miRNA基因表达出最初的pri-miRNA分子。 成熟过程 SiRNA：直接来源是长链的dsRNA(通常为外源);经过Dicer酶*切割形成双链siRNA,而且每个前体daRNA能够被切割成不定数量的siRNA片段。 MiRNA：在细胞核中转录的较大的pri-miRNA经由Drosha(一种RNAse Ⅲ酶)和Pasha(含有双链RNA结合区域)加工成为单链pre-miRNA;接着，发夹状、部分互补的pre
和Hamilton首先发现是~25nt的核糖核酸能特异性抑制具有相应互补序列的基因表达。那么核糖核酸干扰（RNAi）是怎样工作的呢？首先外源导入的双链核糖核酸(dsRNA)被切割为21~23nt的小分子干扰核糖核酸(siRNA)。Dicer, 作为特异性核糖核酸酶家族的一个成员，切割双链核糖核酸为19~23nt小分子干扰核糖核酸（siRNA），这是一个依赖ATP的耗能过程. 切割后的 siRNA具有3'两个核苷酸TT突出末端。然后siRNA结合到核糖核酸酶复合物上形成RNA诱导的基因沉默复合体（RISC，RNA
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