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- 详细信息
- 文献和实验
- 技术资料
- 抗体名:
人类白细胞抗原G抗体
- 抗体英文名:
HLA G
- 靶点:
细胞浆 细胞膜 分泌型蛋白
- 浓度:
1mg/ml
- 应用范围:
Elisa=1:500-1000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500,ICC=1:100-500,
- 宿主:
Rabbit
- 适应物种:
Human,Mouse,Rat,Dog,Pig,Cow,Rabbit,Sheep,Guinea Pig,
- 保质期:
一年
- 抗原来源:
Rabbit
- 目录编号:
TF0752R
- 级别:
I级
- 库存:
10
- 供应商:
晶风生物
- 标记物:
FITC/Alexa/CY357/BIo/HRP
- 克隆性:
Polyclonal
- 保存条件:
-20
- 形态:
Liquid
- 亚型:
IgG
- 免疫原:
KLH conjugated synthetic peptide derived
- 规格:
50ul/100ul/200ul
产品规格:100ul/200ul(部分有50ul,如需更大包装或其他具体规格,请咨询客服)
研究领域:肿瘤 细胞生物 免疫学等
抗体来源:Rabbit
克隆类型:Polyclonal
交叉反应:Human,Mouse,Rat,Dog,Pig,Cow,Rabbit,Sheep,
产品应用:WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 Flow-Cyt=1μg/Test IF=1:100-500 (石蜡切片需做抗原修复)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
性 状:Liquid
浓 度:1mg/ml
免 疫 原:KLH conjugated synthetic peptide derived
亚 型:IgG
纯化方法:affinity purified by Protein A
储 存 液:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件:Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
HLAG人类白细胞抗原G抗体相关抗体示例(非本抗体,如需本抗体,请联系客服索要说明书):
Sample:
Liver (Mouse) Lysate at 40 ug
Spleen (Mouse) Lysate at 40 ug
NIH/3T3 (Mouse) CellLysate at 30 ug
RAW246.7 (Mouse) CellLysate at 30 ug
Primary: Anti- IL12 at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 22 kD
Observed band size: 35/36 kD
paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (IL12) Polyclonal Antibody, Unconjugated at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) instructions and DAB staining.

paraffin embedded (rat brain tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (IL12) Polyclonal Antibody, Unconjugated at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) instructionsand DAB staining.

HLAG人类白细胞抗原G抗体
Blank control (blue line): Mouse spleen (blue).
Primary Antibody (green line): Rabbit Anti- IL12 antibody
Dilution: 1μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody (white blue line): Goat anti-rabbit IgG-FITC
Dilution: 1μg /test.
Protocol
The cells were fixed with 70% ice-cold methanol overnight at 4℃. Cells stained with Primary Antibody for 30 min at room temperature. The cells were then incubated in 1 X PBS/2%BSA/10% goat serum to block non-specific protein-protein interactions followed by the antibody for 15 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.

Tissue/cell: rat colitis tissue; paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,at 37℃ for 20 min;
Incubation: Anti-IL-12 Polyclonal Antibody, Unconjugated 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB staining
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文献和实验人类白细胞抗原(HLA)是分布在组织细胞表面的一组膜蛋白,它们的基本生物学功能是将抗原信息提呈给T细胞识别,从而启动和调节特异性免疫应答。根据分子结构、组织分布和生物学功能的差异,HLA抗原可以分为二类,即HLA-I类抗原(包括HLA-A、B、C)和HLA-II类抗原(HLA-DR、DQ、DP)。HLA抗原由第六染色体短臂上一组紧密连锁的基因群编码。在群体中,HLA抗原及其编码基因均表现出高度的遗传多态性,HLA多态性的检测称为HLA分型(HLA typing)。HLA分型可用于器官
人类白细胞抗原(HLA)基因产物分析被用于临床下列方面: (1)最重要是对脏器或骨髓移植的作用,为移植选择相配对的供、受者, (2)选择HLA匹配的单一供者血小板,用于对混合血小板悬液输注无效的血小板减少症患者, (3)筛选疾病相关的遗传因素医`学教育网搜集整理。 (4)法医学上的应用,亲子鉴定中为有力证据之一。此外,HLA与免疫病理学的相关性也日益密切。与某些疾病相关的T细胞表面抗原鉴定,通过HLA/HLA配体相互作用的免疫效应细胞之间的通讯等,都延伸
本文主要介绍了人类白细胞抗原配型的一些基本问题。具体目录如下: 一、人类白细胞抗原配型 主要组织相容性复合物(MHC)是决定一个器官植入遗传基础不同的个体时,是否排斥的一组基因。人类主要组织相容性复合物的产物成为人类白细胞抗原(HLA)。 1、人类白细胞抗原简介 2、HLA遗传 3、HLA抗原的组织分布 4、HLA抗原频率、单倍型频率和连锁不平衡性 5、HLA发展史 6、HLA的基因结构 7、HLA的配型与骨髓移植 8、HLA分型方法 详情请查看
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