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- 详细信息
- 文献和实验
- 技术资料
- 抗体名:
淋巴细胞特异性蛋白酪氨酸激酶抗体
- 抗体英文名:
Lck
- 靶点:
细胞浆 细胞膜 分泌型蛋白
- 浓度:
1mg/ml
- 应用范围:
Elisa=1:500-1000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500,ICC=1:100-500,
- 宿主:
Rabbit
- 适应物种:
Human,Mouse,Rat,Dog,Pig,Cow,Rabbit,Sheep,Guinea Pig,
- 保质期:
一年
- 抗原来源:
Rabbit
- 目录编号:
TF2649R
- 级别:
I级
- 库存:
10
- 供应商:
晶风生物
- 标记物:
FITC/Alexa/CY357/BIo/HRP
- 克隆性:
Polyclonal
- 保存条件:
-20
- 形态:
Liquid
- 亚型:
IgG
- 免疫原:
KLH conjugated synthetic peptide derived
- 规格:
50ul/100ul/200ul
产品规格:100ul/200ul(部分有50ul,如需更大包装或其他具体规格,请咨询客服)
研究领域:肿瘤 细胞生物 免疫学等
抗体来源:Rabbit
克隆类型:Polyclonal
交叉反应:Human,Mouse,Rat,Dog,Pig,Cow,Rabbit,Sheep,
产品应用:WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 Flow-Cyt=1μg/Test IF=1:100-500 (石蜡切片需做抗原修复)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
性 状:Liquid
浓 度:1mg/ml
免 疫 原:KLH conjugated synthetic peptide derived
亚 型:IgG
纯化方法:affinity purified by Protein A
储 存 液:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件:Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Lck淋巴细胞特异性蛋白酪氨酸激酶抗体相关抗体示例(非本抗体,如需本抗体,请联系客服索要说明书):
Sample:
Liver (Mouse) Lysate at 40 ug
Spleen (Mouse) Lysate at 40 ug
NIH/3T3 (Mouse) CellLysate at 30 ug
RAW246.7 (Mouse) CellLysate at 30 ug
Primary: Anti- IL12 at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 22 kD
Observed band size: 35/36 kD
paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (IL12) Polyclonal Antibody, Unconjugated at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) instructions and DAB staining.

paraffin embedded (rat brain tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (IL12) Polyclonal Antibody, Unconjugated at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) instructionsand DAB staining.

Lck淋巴细胞特异性蛋白酪氨酸激酶抗体
Blank control (blue line): Mouse spleen (blue).
Primary Antibody (green line): Rabbit Anti- IL12 antibody
Dilution: 1μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody (white blue line): Goat anti-rabbit IgG-FITC
Dilution: 1μg /test.
Protocol
The cells were fixed with 70% ice-cold methanol overnight at 4℃. Cells stained with Primary Antibody for 30 min at room temperature. The cells were then incubated in 1 X PBS/2%BSA/10% goat serum to block non-specific protein-protein interactions followed by the antibody for 15 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.

Tissue/cell: rat colitis tissue; paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,at 37℃ for 20 min;
Incubation: Anti-IL-12 Polyclonal Antibody, Unconjugated 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB staining
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文献和实验(src) FcεR Ⅱ(CD23) 肥大细胞、嗜碱性粒细胞 Fyn CD36 血小板 Lyn、Fyn、Yes 一、p56 lck p56 lck 属于以p60v-src/c-src为代表的蛋白酪氨酸激酶家族成员。同这个家族的其它成员一样都具有酪氨酸激酶活性。目前发现,p56 lck 相对特异地存在于淋巴细胞中,尤其是成熟的静止T淋巴细胞中。p56 lck 可能在T
名称的由来。NK 细胞的数量相对于 B 细胞和T细胞来讲非常稀少,但是 NK 细胞具有非常强烈的细胞毒作用(Cytotoxicity),在机体的天然免疫中占据非常重要的地位。 T 细胞主要分泌一些免疫效应分子(如IFN-γ、IL-4、IL-10、IL-17、IL-21等)或者直接对感染或异化的免疫细胞进行杀伤,CD3 是 T 细胞的主要表面标志物;B 细胞是抗体的来源细胞,激活后大量产生抗体的 B 细胞特称为浆细胞,是机体生产抗体的工厂,其常用的表面标志物为 CD19。在流式检测中,我们通常用 CD
蛋白质,尽管这些反应一般要比抗体-抗原间的反应弱,但在待纯化的抗原制品中有过量的非特异性蛋白就将影响纯化效果。 以下几种方法可使非特异性结合降到最低限度。 首先,如同其他亲和层析法一样,所用基质的量必须调整到刚好在其结合纯化制品中所有抗原的水平上,使纯化后的抗原溶液中非特异性蛋白的量尽可能降低。微珠的用量可通过预试确定,并可以测定不同体积的抗体-微珠基质捕获抗原的量。 第二种方法是降低层析柱的非特异性吸附量。许多吸附到柱基质上的蛋白质是部分或全部变性的蛋白。在制备抗原溶液时
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