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- 详细信息
- 文献和实验
- 技术资料
- 抗体名:
肿瘤坏死因子受体相关因子1抗体
- 抗体英文名:
TRAF1
- 靶点:
细胞浆 细胞膜 分泌型蛋白
- 浓度:
1mg/ml
- 应用范围:
Elisa=1:500-1000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500,ICC=1:100-500,
- 宿主:
Rabbit
- 适应物种:
Human,Mouse,Rat,Dog,Pig,Cow,Rabbit,Sheep,Guinea Pig,
- 保质期:
一年
- 抗原来源:
Rabbit
- 目录编号:
TF1212R
- 级别:
I级
- 库存:
10
- 供应商:
晶风生物
- 标记物:
FITC/Alexa/CY357/BIo/HRP
- 克隆性:
Polyclonal
- 保存条件:
-20
- 形态:
Liquid
- 亚型:
IgG
- 免疫原:
KLH conjugated synthetic peptide derived
- 规格:
50ul/100ul/200ul
产品规格:100ul/200ul(部分有50ul,如需更大包装或其他具体规格,请咨询客服)
研究领域:肿瘤 细胞生物 免疫学等
抗体来源:Rabbit
克隆类型:Polyclonal
交叉反应:Human,Mouse,Rat,Dog,Pig,Cow,Rabbit,Sheep,
产品应用:WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 Flow-Cyt=1μg/Test IF=1:100-500 (石蜡切片需做抗原修复)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
性 状:Liquid
浓 度:1mg/ml
免 疫 原:KLH conjugated synthetic peptide derived
亚 型:IgG
纯化方法:affinity purified by Protein A
储 存 液:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件:Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
TRAF1肿瘤坏死因子受体相关因子1抗体相关抗体示例(非本抗体,如需本抗体,请联系客服索要说明书):
Sample:
Liver (Mouse) Lysate at 40 ug
Spleen (Mouse) Lysate at 40 ug
NIH/3T3 (Mouse) CellLysate at 30 ug
RAW246.7 (Mouse) CellLysate at 30 ug
Primary: Anti- IL12 at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 22 kD
Observed band size: 35/36 kD
paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (IL12) Polyclonal Antibody, Unconjugated at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) instructions and DAB staining.

paraffin embedded (rat brain tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (IL12) Polyclonal Antibody, Unconjugated at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) instructionsand DAB staining.

TRAF1肿瘤坏死因子受体相关因子1抗体
Blank control (blue line): Mouse spleen (blue).
Primary Antibody (green line): Rabbit Anti- IL12 antibody
Dilution: 1μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody (white blue line): Goat anti-rabbit IgG-FITC
Dilution: 1μg /test.
Protocol
The cells were fixed with 70% ice-cold methanol overnight at 4℃. Cells stained with Primary Antibody for 30 min at room temperature. The cells were then incubated in 1 X PBS/2%BSA/10% goat serum to block non-specific protein-protein interactions followed by the antibody for 15 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.

Tissue/cell: rat colitis tissue; paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,at 37℃ for 20 min;
Incubation: Anti-IL-12 Polyclonal Antibody, Unconjugated 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB staining
欢迎新老客户咨询订购:TRAF1肿瘤坏死因子受体相关因子1抗体
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文献和实验受体饥饿引起的细胞凋亡 TNFR和Fas介导死亡信号转导的反馈调节 DD:死亡结构域; DED:死亡效应结构域; IKK:IκB激酶; RIP:受体相互作用蛋白; TNFR:肿瘤坏死因子受体结构域; TRAF:TNFR相关因子; FLIP:FLIC抑制蛋白。 图中下部分显示另一条由caspase 9参与的死亡信号途径即线粒体途径。在细胞因子作用下,带有相应受体的免疫细胞可大量扩增而参与应答并分泌效应
一、死亡受体介导的细胞凋亡 死亡受体属于肿瘤坏死因子基因家族,其共同特征是都有相似的、富含半胱氨酸的细胞外结构域。 死亡受体还有一个同源的、称为死亡结构域的胞内序列。死亡结构域一般使死亡受体与胞内凋亡机制相连,但它们有时也会介导与凋亡无关或抑制凋亡的过程。 (一)FasL-Fas 系统介导的细胞凋亡 自杀相关因子Fas(factor associated suicide)是广泛表达于正常细胞和肿瘤细胞膜表面的I型受体,其胞浆段
单链抗体可变区基因片段五聚体作用于凋亡诱导配体受体-2来增强其对癌症的治疗作用
单链抗体可变区基因片段(scFvs) 已被看做是一类有前景的药物。然而,它们的低亲和力和快速的血浆清除率限制了其广泛的临床应用。在本研究中,我们旨在通过多聚scFv提高scFv的抗体表达,再通过与人软骨寡聚基质蛋白(COMP)48的螺旋结构域结合以抵抗死亡受体5(DR5,肿瘤坏死因子10B)。通过合成一种五聚体,我们称其为共同体,在体外和体内实验中,scFv的活性增加了10倍,并且共同体比它的单体配对物在抗肿瘤活性方面更有效。标准的抗体类药物正被成功地用于临床治疗癌症。然而,由于标准抗体的低产
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