- ￥1880
- 晶风生物
- TF0549R
- 中国
- 2025年07月12日
- Elisa=1:500-1000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500,ICC=1:100-500,
- Rabbit
- Human,Mouse,Rat,Dog,Pig,Cow,Rabbit,Sheep,Guinea Pig,
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- 详细信息
- 文献和实验
- 技术资料
- 抗体名：
Ⅲ型胶原蛋白/胶原蛋白3/3型胶原蛋白/III型胶原抗体
- 抗体英文名：
Collagen III
- 靶点：
细胞浆 细胞膜 分泌型蛋白
- 浓度：
1mg/ml
- 应用范围：
Elisa=1:500-1000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500,ICC=1:100-500,
- 宿主：
Rabbit
- 适应物种：
Human,Mouse,Rat,Dog,Pig,Cow,Rabbit,Sheep,Guinea Pig,
- 保质期：
一年
- 抗原来源：
Rabbit
- 目录编号：
TF0549R
- 级别：
I级
- 库存：
10
- 供应商：
晶风生物
- 标记物：
FITC/Alexa/CY357/BIo/HRP
- 克隆性：
Polyclonal
- 保存条件：
-20
- 形态：
Liquid
- 亚型：
IgG
- 免疫原：
KLH conjugated synthetic peptide derived
- 规格：
50ul/100ul/200ul
产品规格：100ul/200ul(部分有50ul，如需更大包装或其他具体规格，请咨询客服)
研究领域：肿瘤 细胞生物 免疫学等
抗体来源：Rabbit
克隆类型：Polyclonal
交叉反应：Human,Mouse,Rat,Dog,Pig,Cow,Rabbit,Sheep,
产品应用：WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 Flow-Cyt=1μg/Test IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
性 状：Liquid
浓 度：1mg/ml
免 疫 原：KLH conjugated synthetic peptide derived
亚 型：IgG
纯化方法：affinity purified by Protein A
储 存 液：0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件：Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
CollagenIIIⅢ型胶原蛋白/胶原蛋白3/3型胶原蛋白/III型胶原抗体相关抗体示例（非本抗体，如需本抗体，请联系客服索要说明书）：
Sample:
Liver (Mouse) Lysate at 40 ug
Spleen (Mouse) Lysate at 40 ug
NIH/3T3 (Mouse) CellLysate at 30 ug
RAW246.7 (Mouse) CellLysate at 30 ug
Primary: Anti- IL12 at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 22 kD
Observed band size: 35/36 kD
paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (IL12) Polyclonal Antibody, Unconjugated at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) instructions and DAB staining.
paraffin embedded (rat brain tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (IL12) Polyclonal Antibody, Unconjugated at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) instructionsand DAB staining.
CollagenIIIⅢ型胶原蛋白/胶原蛋白3/3型胶原蛋白/III型胶原抗体
Blank control (blue line): Mouse spleen (blue).
Primary Antibody (green line): Rabbit Anti- IL12 antibody
Dilution: 1μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody (white blue line): Goat anti-rabbit IgG-FITC
Dilution: 1μg /test.
Protocol
The cells were fixed with 70% ice-cold methanol overnight at 4℃. Cells stained with Primary Antibody for 30 min at room temperature. The cells were then incubated in 1 X PBS/2%BSA/10% goat serum to block non-specific protein-protein interactions followed by the antibody for 15 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
Tissue/cell: rat colitis tissue; paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,at 37℃ for 20 min;
Incubation: Anti-IL-12 Polyclonal Antibody, Unconjugated 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB staining
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文献和实验良好优势。但因以单束固定波长的激光束扫描，为了使激光扫到整个生物芯片，需要激光头，或生物芯片的二维机械运动，扫描效率则比CCD型低。针对这个问题，本文提出了一种新型的以振镜和远心f-θ物镜（线性成像物镜）结合实现X方向扫描，机械式Y方向线性扫描相结合的光机二维扫描技术。一、共焦生物芯片扫描仪的共焦原理与扫描实现方法激光共聚焦生物芯片扫描仪采用共聚焦成像原理，使点源、样品及光阑处于彼此对应的共轭位置，原理图如图1所示。激光器发出的激光经分光镜和物镜后，激发生物芯片上的荧光分子发光，部分球状散射的荧
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1 signaling dictates pancreatic cancer outcome。 该研究首次揭示胰腺导管腺癌 ECM 中 I 型胶原蛋白（Col I）的降解状态决定了病理发展和患者的生存期，切割形式的 Col I 促进肿瘤代谢和胰腺导管腺癌发展，而全长形式的 Col I 则靶向干预 DDR1-NRF2 通路能显著抑制肿瘤发生。 图 3：来源 Nature 4. Science：揭示+1 核小体调控转录起始的分子机制 此前，+1 核小体与转录起始复合物之间的结构功能关联性不明。 2022
6 胶原的右手超螺旋结构 胶原蛋白的氨基酸组成有如下特征：①甘氨酸几乎占总氨基酸残基的三分之一，即每隔两个其他氨基酸残基(X，Y)即有一个甘氨酸，故其肽链可用(甘－X－Y)n来表示。②含有较多在其他蛋白质中少见的羧脯氨酸和羧赖氨酸残基，也有较多脯氨酸(pro)和赖氨酸。如脯氨酸(Pro)和4―羟脯氨酸(4―hydroxyproline,Hyp)含量高达15～30%。同时还含有少量3―羟脯氨酸(3hydroxyproline)和5―羟赖氨酸(5―hydroxylysine,Hyl)。羟脯
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