For efficient transfection of primary mouse neural stem cells in the NucleofectorTM 2b Device
Product Overview
The NucleofectorTM 2b Device (or older generations I or II) works cell type specific kits, each of them dedicated to an individual primary cell. This NucleofectorTM Kit is the optimal kit for efficient transfectiion of primary mouse neural stem cells (NSCs).
The respective cell type-specific Optimized Protocol or recommendation is available under "Instructions" or in our knowledge database. In this Optimized Protocol the best Nucleofection conditions are indicated. In addition, we share our experience and knowledge for treatment of this cell type.
The kit is available in different variants for either 10 or 25 or 4 x 25 reactions.
Benefits
High transfection efficiencies for primary cells
Cells maintain functionality post transfection
Applications
Transfection of cell numbers in the range of 2 × 105 to 2 × 107 cells
Low-throughput transfection in single cuvette format
Content & Storage
Content
4 x 2.25 mL NucleofectorTM Solution for Mouse Neural Stem Cells 4 x 0.5 mL Supplement 1 4 x 30 µg pmaxGFPTM Vector (0.5 μg/μl in 10 mM Tris pH 8.0) 4 x 25 Aluminum cuvette (100 µL) 4 x 25 Single use pipettes