Recombinant Mouse Siglec-15 (C-6His)(CW71)
产品说明(Description)
Recombinant Mouse Sialic Acid-binding Ig-like Lectin 15 is produced by our Mammalian expression system and the target gene encoding Arg24-Thr262 is expressed with a 6His tag at the C-terminus.
Accession #: A7E1W8
Known as:Angiopoietin-related protein 4; 425O18-1; Angiopoietin-like protein 4; Fasting-induced adipose factor; Hepatic fibrinogen/angiopoietin-related protein; HFARP; Secreted protein Bk89; Angptl4; Farp; Fiaf; Ng27
制剂(Formulation)
Lyophilized from a 0.2 μm filtered solution of PBS, 150mMnacl, 5%Thehalose, 0.3%chaps, Ph7.4.
质量控制(Quality Control)
Purity: Greater than 95% as determined by reducing SDS-PAGE.
Endotoxin: Less than 0.1 ng/ug (1 IEU/ug) as determined by LAL test.
Bioactivity-Elisa: |
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Bioactivity-BLI: |
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Immobilized Mouse Siglec-15-His (Cat#CW71) at 2μg/ml (100 μl/well) can bind Anti-Human Siglec15 mAb. The ED50 of Anti-Human Siglec15 mAb is 27.7 ng/ml. |
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Loaded Anti-Human Siglec15 mAb-mFc(Cat#NC007) on AMQ Biosensor, can bind Mouse Siglec-15-His(Cat#CW71) with an affinity constant of 0.33 nM as determined in BLI assay. |
复溶(Reconstitution)
Always centrifuge tubes before opening. Do not mix by vortex or pipetting.
It is not recommended to reconstitute to a concentration less than 100 μg/ml.
Dissolve the lyophilized protein in distilled water.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
保存(Storage)
Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks.
Reconstituted protein solution can be stored at 4-7°C for 2-7 days.
Aliquots of reconstituted samples are stable at < -20°C for 3 months.
背景(Background)
Human Siglec-15 is a transmembrane glycoprotein in the Siglec family. Siglecs are type I transmembrane proteins where the NH3+-terminus is in the extracellular space and the COO?-terminus is cytosolic. Each Siglec contains an N-terminal V-type immunoglobulin domain (Ig domain) which acts as the binding receptor for sialic acid. These lectins are placed into the group of I-type lectins because the lectin domain is an immunoglobulin fold. All Siglecs are extended from the cell surface by C2-type Ig domains which have no binding activity. Siglecs differ in the number of these C2-type domains. Human Siglec-15 consists of a 244 amino acid (aa) extracellular domain (ECD) with two Ig-like domains, a 21 aa transmembrane segment, and a 44 aa cytoplasmic domain. Siglec-15 function is important for osteoclast formation and TRANCE/RANK Ligand signaling in osteoclasts
电泳(SDS-PAGE)
FOR RESEARCH USE ONLY.