Recombinant Human CD3E (C-mFc)

Recombinant Human CD3E (C-mFc) (C00E)

 

产品说明(Description)

Recombinant Human T-Cell Surface Glycoprotein CD3 Epsilon Chain is produced by our Mammalian expression system and the target gene encoding Gln22-Thr48 is expressed with a mFc tag at the C-terminus.

Accession #: P07766

Known as: T-Cell Surface Glycoprotein CD3 Epsilon Chain; T-Cell Surface Antigen T3/Leu-4 Epsilon Chain; CD3e; CD3E; T3E

 

制剂(Formulation)

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

 

质量控制(Quality Control)

Purity:          Greater than 95% as determined by reducing SDS-PAGE.  

Endotoxin:   Less than 0.1 ng/µg (1 EU/µg) as determined by LAL test.

 

Bioactivity:		Bioactivity-BLI:
Immobilized  Human  CD3E-mFc(Cat#C00E) at 10μg/ml (100 μl/well) can bind Human Anti-CD3(Cat#GMP-A018).  The ED50 of Human CD3E-mFc(Cat#C00E) is 9.4 ng/ml.		Loaded Anti-Human/Monkey CD3E mAb-Fc(Cat#NC006) on AHC Biosensor, can bind  Human CD3E-mFc(Cat#C00E) with an affinity constant of <10-3 nM as determined in BLI assay.

 

复溶(Reconstitution)

Always centrifuge tubes before opening. Do not mix by vortex or pipetting.
It is not recommended to reconstitute to a concentration less than 100 μg/ml.
Dissolve the lyophilized protein in distilled water.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

 

保存(Storage)

Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks.

Reconstituted protein solution can be stored at 4-7°C for 2-7 days.

Aliquots of reconstituted samples are stable at < -20°C for 3 months. 

 

背景(Background)

T-Cell Surface Glycoprotein CD3 ε Chain (CD3ε) is a single-pass type I membrane protein. CD3ε contains 1 Ig-like (immunoglobulin-like) domain and 1 ITAM domain. CD3ε is a polypeptide encoded by the CD3E gene on chromosome 11 in humans. The T cell receptor-CD3 complex (TCR/CD3 complex) is involved in T-cell development and several intracellular signal-transduction pathways. This complex is critical for T-cell development and function, and represents one of the most complex transmembrane receptors. The T cell receptor-CD3 complex is unique in having ten cytoplasmic immunoreceptor tyrosine-based activation motifs (ITAMs). TCR/CD3 complex plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways.

 

电泳(SDS-PAGE)

 

FOR RESEARCH USE ONLY.