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- 详细信息
- 文献和实验
- 技术资料
- 英文名:
His Tag
- 保质期:
3年
- 保存条件:
-20度
- 库存:
大量
- 供应商:
中科亚光
- 规格:
mg
序列信息:HHHHHH
分子式:840.85
分子量:C36H44N18O7 g/mol
CAS No. : 64134-30-1
别名:His-tag 标签多肽,His6
介绍:Hexa-His 是由 6 个组氨酸构成的六肽,常作为融合蛋白的金属结合位点。
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文献和实验solubilizing agents, having frequently been observed to improve the yield, enhance the solubility, and promote the proper folding of its fusion partners. The use of a dual hexahistidine–maltose-binding protein affinity tag (His6 –MBP) has the additional
Purification and Characterization of His-Tagged Antibody Fragments
, typically His6 , fused to the recombinant protein. In the case of antibody fragments that are composed of different chains, e.g., Fv and Fab, the site of attachment for the His6 tag has to be carefully chosen, and mild chromatographic conditions
A uniform procedure for the purification of CDK7/CycH/MAT1, CDK8/CycC and CDK9/CycT1
We have established a uniform procedure for the expression and purification of the cyclin-dependent kinases CDK7/CycH/MAT1, CDK8/CycC and CDK9/CycT1. We attach a His6 -tag to one of the subunits of each complex and then co-express
技术资料







