科学家眼中的Addexbio，那些你不知道的事儿

细胞订购选择孚博生物，科研项目快人一步！欢迎广大科研、企业单位老师们咨询订购！

 

AddexBio Technologies的历史和发展

AddexBio是一家为学术界、制药和生物技术行业提供并研发优质产品和服务的全球供应商，并致力于为科学界提供生物素化试剂、细胞系服务、细胞系产品、荧光标记肽和研究试剂。

经过十年以上的发展，AddexBio已成为一家能提供真正物美价廉的产品的企业。其细胞系产品质量可以媲美ATCC，其他产品也经过严格的质控，客户可以放心使用。其强劲价格优势更让AddexBio的产品成为众多科研工作者的优先选择之一。
 

AddexBio的细胞产品有哪些优势？

AddexBio提供多种细胞系：用于研究的癌症细胞系、永生化细胞系、原代细胞系和稳定细胞系。价格非常实惠，并且质量控制严格，已受众多科研工作者的力荐。

比如永生化细胞，同样的质量，我们可以用其他大厂不到1/3的价格获取到。

厂家的产品内容详实，直观方便。

此外，厂家的服务也很到位，态度友好、尽职尽责。
 

AddexBio的细胞质检很严苛？

细菌和真菌测试

支原体筛查

病原体检测

细胞系认证

STR鉴定

细胞的出库标准信息官网可查，非常简便。
 
细胞产品示例：
Cell Line Designation: MIN-6
AddexBio Catalog No. C0018008
Cell Line Description:
Disease: Insulinoma (SV40 T-antigen induced cell immortalization)
Origin: Pancreatic Islets
Species: Mus musculus
Tissue: Pancreas
Properties: Adherent; β cells
Recommended Culture Flask: BD Biosciences Tissue Culture-treated flask
Complete Medium: AddexBio Advanced Medium (C0003-04) + 15% FBS + 0.05 mM 2-mercaptoethanol (Invitrogen Cat: 21985023). Please note: in order to allow successful cell attachment to the flask, use ONLY new batch of Advanced Medium and other components. Other components should be added freshly to make the complete medium before use. Complete medium stored in the refrigerator for an extended period of time (> 1-2 weeks) may lose efficacy to maintain cell attachment and cell growth, and therefore, it should be discarded.
Subculture Procedure: 1:2 to 1:3 (80% confluency) using 0.25% trypsin or trypsin/EDTA, 5% CO2; 37°C
Medium Renewal: Two to three times weekly.
Freezing Medium: Culture medium supplemented with 20% (v/v) FBS and 7.5% (v/v) DMSO
Biosafety Level: II
Appropriate safety procedures should always be used with this material. laboratory safety is discussed in the following publication: Biosafety in Microbiological and Biomedical Laboratories, 5th ed. HHS Publication No. (CDC) 93-8395. U.S. Department of Health and
Human Services, Centers for Disease Control and Prevention. Washington DC: U.S.
Government Printing Office; 2007. The entire text is also available online at
www.cdc.gov/od/ohs/biosafty/bmbl4/bmbl4toc.htm
Use Restrictions: These cells are distributed for research purposes only. Addexbio
does not recommend third party distribution of this cell line, as this practice has resulted in the
unintentional spreading o f contaminated cell lines.
Handling Cells Upon Arrival:
Frozen cells must be thawed immediately upo n receipt and grown according to the handling
procedures described here in this instruction manual to ensure the best cell viability.
Note: Avoid refreezing or repetitive freezing cells upon receipt as it may result in irreversible
damage to the cell line.
Disclaimer
Handling Procedure for Frozen Cells:
: We cannot guarantee cell viability if the cells are not thawed immediately upon
receipt and grown according to handling procedures described in this instruction manual.
To insure the highest level of viability, thaw the vial and initiate the culture as soon as possible
upon receipt. If upon arrival, continued storage of the frozen culture is necessary, it should be
stored in liquid nitrogen vapor phase and not at -70°C. Storage at -70°C will result in loss of
viability.
Safety Precaution:
Addexbio highly recommends that protective gloves and clothing always be used and a full face
mask always be worn when handling frozen vials. It is important to note that some vials leak
when submersed in liquid nitrogen and will slowly fill with liquid nitrogen. Upon thawing, the
conversion of the liquid nitrogen back to its gas phase may result in the vessel exploding or
blowing off its cap with dangerous force creating flying debris.
1. Thaw the vial by gentle agitation in a 37°C water bath. To reduce the possibility of
contamination, keep the O-ring and cap out of water. Thawing should be rapid
(approximately 2 minutes).
2. Remove the vial from the water bath as soon as the contents are thawed, and decontaminate by
dipping in or spraying with 70% ethanol. All of the operations from this point on should be
carried out under strict aseptic conditions.
3. Transfer the vial content to a sterile T25 flask containing 9.0 mL complete culture medium.

4. It is important to avoid excessive alkalinity of the medium during recovery of the cells. It is suggested that, prior to the addition of the vial contents, the culture vessel containing the complete growth medium be placed into the incubator for at least 15 minutes to allow the medium to reach its normal pH (7.0-7.6).
5. Incubate the culture at 37°C in a suitable humidified incubator. A 5% CO2 in air atmosphere is recommended. It is recommended to allow the cells to grow without disturbing them for 2-3 days. Try to avoid shaking of the flask when handling.
References for MIN-6 cells:
1. Ishihara, H., Asano, T., Tsukuda, K. et al. (1993). Pancreatic beta cell line MIN6 exhibits characteristics of glucose metabolism and glucose-