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- 库存：
1000
- 供应商：
N/A
- 英文名：
FibrOut™
- 规格：
1ml(500X)
FibrOut™成纤维抑制剂 （原代细胞培养推荐★★★★★）
产品介绍
CHI Scientific研发的成纤维细胞生长抑制体系—FibrOut™，包含多种生化成分和有机试剂，能够抑制成纤维细胞在原代细胞培养中的成长，进而促进靶细胞生长。每一种成纤维细胞抑制剂是组织和细胞特异性的，适用于相应的PrimaCellTM原代细胞培养体系，可以为您提供理想状态的原代细胞。
规格型号
产品体系
人成纤维抑制剂
小鼠成纤维抑制剂
大鼠成纤维抑制剂
禽类成纤维抑制剂
质量检测
每1ml FibrOut™可以混合于500ml培养基，每批试剂均经过成纤维细胞抑制性能测试。
论文引用
Companion-Probe & Race platform for interrogating nuclear protein and migration of living cells
Hong Sun 1, Zaizai Dong 2, Qingyang Zhang 3, Bing Liu 4, Shi Yan 4, Yusen Wang 1, Dedong Yin 5, Peigen Ren 6, Nan Wu 4, Lingqian Chang 7
Biosens Bioelectron. 2022 Aug 15;210:114281. DOI: 10.1016/j.bios.2022.114281
.....with 15% FBS and FibrOutTM fibroblast inhibitor (4-21564, CHI Scientific) (Hang et al., 2022). The above cells were all cultured in a cell incubator (CCL-170JB-8, ESCO) with the condition of 37 ◦C and 5% CO2.
更多：
1)[HTML] CD47 Promotes Age-Associated Deterioration in Angiogenesis, Blood Flow and Glucose Homeostasis.
Kedar Ghimire, * Yao Li, etc. al. Cells. 2020 Jul; 9(7): 1695
….. Mouse FibrOut 11 and Human FibrOut custom prepared for Blood Vessels and Endothelial Tissues were from Chi Scientific Inc. (Maynard,
MA, USA)….. Mouse FibrOut 11 and Human FibrOut custom prepared for blood vessels and endothelial tissues was added to the cell media following the manufacturers’ protocol to suppress fibroblast growth.
2)[HTML] Cancer/testis antigens expression during cultivation of melanoma and soft tissue sarcoma cells
A Danilova, V Misyurin, A Novik, D Girdyuk… - Clinical Sarcoma …, 2020 - Springer
… When cells reached sub-confluence, they were dispersed with 0.25% trypsin–0.02% EDTA
(Sigma-Aldrich, USA) and seeded in a new culture plate. Fibroblast growth-inhibitory cocktail
Human FibrOut™ 9 (Chi Scientific Inc., USA) was used for fibroblast growth prevention …
3) Engineering a Bioartificial Human Colon Model Through Decellularization and Recellularization
HJ Chen, ML Shuler - Cancer Driver Genes, 2019 - Springer
… 5. Media with fibroblast inhibitory reagent: Colonic epithelial cell growth medium with 1%
fibroblast inhibitory reagent (Human Colon FibrOut™ from CHI Scientific) for the first two to three
passages of primary cultures to prevent the outgrowth of fibroblasts. 6 …
4) A pumpless body-on-a-chip model using a primary culture of human intestinal cells and a 3D culture of liver cells
HJ Chen, P Miller, ML Shuler - Lab on a Chip, 2018 - pubs.rsc.org
… ng ml −1 R-spondin 1 (R&D systems) and 50 ng ml −1 Noggin (Peprotech, NJ) in collagen-I coated
flasks (BD scientific, NJ) and incubated in a humidified incubator at 5% CO 2 and 37 °C. After
48 hour culture, fibroblast inhibitory reagent (Human Colon FibrOut™, CHI Scientific …
5) [PDF] A novel way of harvesting a large population of endothelial cells for clinical and experimental use
AO Ilesanmi - Biomed Res Rev, 2018 - pdfs.semanticscholar.org
… If it is eventually decided that fibroblasts should be eliminated completely, there is a commercial
product, the Human Vascular Endothelial PrimaCell™ system that includes an effective fibroblast
elimination system, Vascular Endothelial FibrOut™ (www.chiscientific.com) …
6) [PDF] gene KIF23 in a mouse model of lung adenocarcinoma. Oncogene, 36 (1), pp. 110-121.(doi: 10.1038/onc. 2016.181) This is the author's final accepted version …
F Iltzsche - core.ac.uk
… ml fresh media. Tissue was broken up by pipetting and the cell suspension was cultured in media
supplemented with Mouse FibrOut™ 9 (CHI Scientific). Cells were cultured until confluency was
reached, genotyped and stored for further processing. Antibodies …
7) Very long chain fatty acid β-oxidation in astrocytes: contribution of the ABCD1-dependent and-independent pathways
M Morita, S Shinbo, A Asahi… - Biological and …, 2012 - jstage.jst.go.jp
… The MessageMuter shRNAi Production kit was from EPICENTRE. FibrOut (System 4,
Mouse) was purchased from CHI Scientific (Maynard, MA, USA). Antimycin A and ro-
tenone were purchased from Sigma-Aldrich (St. Louis, MO, USA) …
8) Requirement of the NF-κB subunit p65/RelA for K-Ras–induced lung tumorigenesis
DS Bassères, A Ebbs, E Levantini, AS Baldwin - Cancer research, 2010 - AACR
9) p38α MAP kinase is essential in lung stem and progenitor cell proliferation and differentiation
JJ Ventura, S Tenbaum, E Perdiguero, M Huth… - Nature …, 2007 - nature.com
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文献和实验1) [HTML] Cancer/testis antigens expression during cultivation of melanoma and soft tissue sarcoma cells
A Danilova, V Misyurin, A Novik, D Girdyuk… - Clinical Sarcoma …, 2020 - Springer… When cells reached sub-confluence, they were dispersed with 0.25% trypsin–0.02% EDTA
(Sigma-Aldrich, USA) and seeded in a new culture plate. Fibroblast growth-inhibitory cocktail
Human FibrOut™ 9 (Chi Scientific Inc., USA) was used for fibroblast growth prevention …
2) Engineering a Bioartificial Human Colon Model Through Decellularization and Recellularization
HJ Chen, ML Shuler - Cancer Driver Genes, 2019 - Springer… 5. Media with fibroblast inhibitory reagent: Colonic epithelial cell growth medium with 1%
fibroblast inhibitory reagent (Human Colon FibrOut™ from CHI Scientific) for the first two to three
passages of primary cultures to prevent the outgrowth of fibroblasts. 6 …
3) A pumpless body-on-a-chip model using a primary culture of human intestinal cells and a 3D culture of liver cells
HJ Chen, P Miller, ML Shuler - Lab on a Chip, 2018 - pubs.rsc.org… ng ml −1 R-spondin 1 (R&D systems) and 50 ng ml −1 Noggin (Peprotech, NJ) in collagen-I coated
flasks (BD scientific, NJ) and incubated in a humidified incubator at 5% CO 2 and 37 °C. After
48 hour culture, fibroblast inhibitory reagent (Human Colon FibrOut™, CHI Scientific …
4) [PDF] A novel way of harvesting a large population of endothelial cells for clinical and experimental use
AO Ilesanmi - Biomed Res Rev, 2018 - pdfs.semanticscholar.org… If it is eventually decided that fibroblasts should be eliminated completely, there is a commercial
product, the Human Vascular Endothelial PrimaCell™ system that includes an effective fibroblast
elimination system, Vascular Endothelial FibrOut™ (www.chiscientific.com) …
5) [PDF] gene KIF23 in a mouse model of lung adenocarcinoma. Oncogene, 36 (1), pp. 110-121.(doi: 10.1038/onc. 2016.181) This is the author's final accepted version …
F Iltzsche - core.ac.uk… ml fresh media. Tissue was broken up by pipetting and the cell suspension was cultured in media
supplemented with Mouse FibrOut™ 9 (CHI Scientific). Cells were cultured until confluency was
reached, genotyped and stored for further processing. Antibodies …
6) Very long chain fatty acid β-oxidation in astrocytes: contribution of the ABCD1-dependent and-independent pathways
M Morita, S Shinbo, A Asahi… - Biological and …, 2012 - jstage.jst.go.jp… The MessageMuter shRNAi Production kit was from EPICENTRE. FibrOut (System 4,
Mouse) was purchased from CHI Scientific (Maynard, MA, USA). Antimycin A and ro-
tenone were purchased from Sigma-Aldrich (St. Louis, MO, USA) …
7) Requirement of the NF-κB subunit p65/RelA for K-Ras–induced lung tumorigenesis
DS Bassères, A Ebbs, E Levantini, AS Baldwin - Cancer research, 2010 - AACR8) p38α MAP kinase is essential in lung stem and progenitor cell proliferation and differentiation
JJ Ventura, S Tenbaum, E Perdiguero, M Huth… - Nature …, 2007 - nature.com
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