应用: DNA normalization技术是一种新型的高效平衡化未克隆的全长富集cDNA的有效方法。Trimmer-2试剂盒是Evrogen公司独有产品,利用DNA normalization技术,高效降低高丰度表达基因、富集低丰度表达基因,得到的cDNA包含相等浓度的不同基因,可应用于cDNA文库的构建和直接测序,以及在后续的测序平台Illumina/Solexa、ABI / SOLiD和Roche / 454上的高通量测序。另外,这个试剂盒还与构建cDNA的ClontechSMART-based kit兼容,来用于cDNA均一化。
特点:
l 快速有效地降低在文库中高拷贝存在的cDNA 的丰度
l 克隆文库前对全长丰富的cDNA进行均一化
l 操作简单,无需物理分离步骤
l 与Illumina /Solexa,,ABI / SOLiD和Roche / 454的测序实验完全相容
品牌
货号
品名
规格
Evrogen
NK003
Trimmer-2 cDNA normalization kit
For 10 reactions
典型结果图:
(A) Agarose/EtBr gel-electrophoresis of non-normalized (1) and normalized (2) human cDNA samples;
(B) concentration of abundant transcripts in these samples revealed by Virtual Northern blot. GPDH – glyceraldehyde-3-phosphate dehydrogenase; UBC – ubiquitin C; M, 1-kb DNA size markers (SibEnzyme).
(C) Normalization significantly increases gene discovery rate of cDNA library. Transcript distribution in standard and normalized cDNA libraries from Aplysia neurons: yellow – all sequences, blue – unique sequences; green – non-unique sequences