该产品被引用文献: 1、Researchers used the Direct-zol RNA MiniPrep for heterologous E. coli expression of the FnCpf1 locus by means of RNA-seq and discovered a new endonuclease, Cpf1, which further improves upon the widely used CRISPR-Cas9 genome editing system. Cpf1 targets distinct PAM sequences, requires no tracrRNA, and cleaves DNA with staggered overhangs. Zetsche B, et. al. (2015). Cpf1 is a Single RNA-Guided Endonuclease of a Class 2 CRISPR-Cas System. Cell. 163(3): 759-71. https://www.ncbi.nlm.nih.gov/pubmed/26422227
2、The Direct-zol RNA Miniprep kit was used to isolate pure total RNA from Rat cells, and the high quality RNA was used to create a cDNA library for RT-qPCR analysis of several genes related to hematopoietic Stem Cell (HSC) Development. The consistent RNA extraction allowed the scientists to determine that HSC cells do not significantly contribute to kidney repair following acute kidney injuries. Burst V. et al. (2012) Survival and distribution of injected haematopoietic stem cells in acute kidney injury. Nephrol Dial Transplant 0: 1–8 https://www.ncbi.nlm.nih.gov/pubmed/23197679
3、High-quality RNA isolated with the Direct-zol™ RNA MiniPrep from fecal and culture samples were shown to be instrumental in the development of a rotavirus early detection system using RT-PCR. The efficient RNA isolation helps in identification of severe gastroenteritis in infants and young children. Yoshiki F, Takashi S, Takagi H, et al. Amplification of all 11 RNA segments of group A rotaviruses based on reverse transcription polymerase chain reaction Microbiol Immunol 2012; 56: https://www.ncbi.nlm.nih.gov/pubmed/22708835
4、Total RNA was extracted from human, rat, and mouse cortical neurons using the Direct-zol RNA MiniPrep Kit. The high-quality RNA was reverse transcribed into cDNA and used to investigate the effect of BPA exposure on neurodevelopment by real-time PCR analysis. Yeo M, et. al. (2013) Bisphenol A delays the perinatal chloride shift in cortical neurons by epigenetic effects on the Kcc2 promoter. Proc Natl Acad Sci U S A. 110(11):4315-20. https://www.ncbi.nlm.nih.gov/pubmed/23440186