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三色预染蛋白-Marker-(10-245KD)

  • 价  格: ¥50
  • 保存条件: -20℃
  • 供  应  商: 上海万生昊天生物技术有限公司
  • 保  质  期: 12个月
  • 试用规格: 15ul
  • 产  地: USA
  • 货  号: MKP024
  • 品  牌: wshtbio
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已有 31 人申请试用(待审核)
上海万生昊天生物技术有限公司
商家诚信度:
成立时间:2008
入驻丁香通年限:4年
商家等级:金牌客户
产品信息完整度:65.0%
联系人:史先生 点击这里给我发消息

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电话:021-50917892

邮件:info@wshtbio.com

手机:15026523802

地址:上海市闵行区浦江镇江凯路199号1号楼810室

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* 试用品 三色预染蛋白 Marker (10-245KD) (31 人申请)
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试用说明

试用申请时间: 2019年08月07日— 2019年08月25日 1. 运费:不包邮
2. 发货时间:8.25审核通过后统一发放
3. 申请要求:提供单位,实验名称,申请理由
4. 其他说明:

产品详情

规格: 15ul 产品价格: ¥50.0
本次活动产品规格为15ul/管。

三色预染蛋白marker(10-245),5 μl per well for 100 applications。


Storage:
4°C for 3 months
-20°C for 24 months

Loading Volume:
 3 μl or 5 μl per loading for clear visualization during electrophoresis on a 15-well or 10-well mini-gel.
 1.5~2.5 μl per well for general Western blotting transfer.
 Apply more for a thicker (> 1.5 mm) or larger gel.

Description:
The 3-color High Range Protein Marker is a ready to use three-color protein standard with 12 pre-stained proteins covering a wide range of molecular weights from 10 to 245 kDa in Tris-Glycine buffer (9 to 235 kDa in Bis-Tris (MOPS) buffer and 10-235 kDa in Bis-Tris (MES) buffer). Proteins are covalently coupled with different chromophores for easy identification of bands, with two reference proteins carrying enhanced intensity corresponding to a green at 25 kDa and red at 75 kDa, respectively, as separated on SDS-PAGE (Tris-Glycine buffer).

The 3-color High Range Protein Marker is designed for monitoring protein separation during SDS-polyacrylamide gel electrophoresis, verification of Western transfer efficiency on membranes (PVDF, nylon, or nitrocellulose) and for approximating the size of proteins.

The marker is supplied in gel loading buffer and is ready to use. Do NOT heat, dilute, or add reducing agents before loading.

Contents:
Approximately 0.1~0.4 mg/ml of each protein in the buffer (20 mM Tris-phosphate (pH 7.5 at 25°C), 2 % SDS, 0.2 mM Dithiothreitol, 3.6 M Urea, and 15 % (v/v) Glycerol).

Guide for Molecular Weight Estimation:
Migration patterns and approximate MWs (kDa) of 3-color High Range Protein Marker in different electrophoresis conditions are listed below:

 
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