nmda receptor, nr1 subunit, n1 splice variant insert抗体
- 参考价格： ¥3685.0
- 品 牌： abgent
- 货 号： AN1051
- 适应物种： 小鼠,大鼠
- 应用范围： 免疫印迹
- 抗体类型： 一抗
- 海 运： Blue Ice
- 基因id： 24408
- 详细信息
- 图片
详细信息
产品概览
| 背景： | The ion channels activated by glutamate that are sensitive to N-methyl-D-aspartate (NMDA) are designated NMDA Receptors (NMDAR). The NMDAR plays an essential role in memory, neuronal development and it has also been implicated in several disorders of the central nervous system including Alzheimer’s, epilepsy and ischemic neuronal cell death (Grosshans et al., 2002; Wenthold et al., 2003; Carroll and Zukin, 2002). There are a number of different splice variants of the NR1-Subunit (Foldes et al., 1994; Zukin and Bennett, 1995). Differential splicing of three exons in the NR1-Subunit generates up to eight NR1-Subunit splice variants and 7 of these have been identified in cDNA libraries. These exons encode a 21 amino acid N-terminal domain (N1) and adjacent sequences in the C-terminus (C1 and C2). Splicing out the C2 cassette eliminates the first stop codon and produces a new reading frame that generates a new sequence of 22 amino acids (C2'). Considerable attention has been focused on the distribution and expression of these splice variants that may affect the functional properties and regulation of the NMDAR.REFERENCESCarroll RC, Zukin RS (2002) NMDA-receptor trafficking and targeting: implications for synaptic transmission and plasticity. Trends Neurosci 25:571-577.Foldes RL, Rampersad V, Kamboj RK (1994) Cloning and sequence analysis of additional splice variants encoding human N-methyl-D-aspartate receptor (hNR1) subunits. Gene 147:303-304.Grosshans DR, Clayton DA, Coultrap SJ, Browning MD (2002) LTP leads to rapid surface expression of NMDA but not AMPA receptors in adult rat CA1. Nat Neurosci 5:27-33.Wenthold RJ, Prybylowski K, Standley S, Sans N, Petralia RS (2003) Trafficking of NMDA receptors. Annu Rev Pharmacol Toxicol 43:335-358.Zukin RS, Bennett MVL (1995) Alternatively spliced isoforms of the NMDARI receptor subunit. Trends Neurosci 18:306-313.Note: Dr. Michael Browning, a co-author of one of the cited papers, is President and founder of PhosphoSolutions |
| 其他名字： | Glutamate receptor ionotropic, NMDA 1, GluN1, Glutamate [NMDA] receptor subunit zeta-1, N-methyl-D-aspartate receptor subunit NR1, NMD-R1, Grin1, Nmdar1 |
产品特性
| 抗体特异性： | Specific for the ~120k NR1 subunit, N1 splice variant insert protein of the NMDA receptor. Does not recognize NR1 subunits of the NMDA receptor that do not contain the N1 insert. |
| 预测型适应物种： | Human, Chicken, Monkey, Xenopus |
靶点信息
| 基因名： | GRIN1 |
应用与保存
| 稀释浓度： | WB~~ 1:1000 |
| 注意事项： | NMDA Receptor, NR1 Subunit, N1 Splice Variant Insert Antibody is for research use only and not for use in diagnostic or therapeutic procedures. |
其他
| 参考文献： | Carroll RC, Zukin RS (2002) NMDA-receptor trafficking and targeting: implications for synaptic transmission and plasticity. Trends Neurosci 25:571-577.Foldes RL, Rampersad V, Kamboj RK (1994) Cloning and sequence analysis of additional splice variants encoding human N-methyl-D-aspartate receptor (hNR1) subunits. Gene 147:303-304.Grosshans DR, Clayton DA, Coultrap SJ, Browning MD (2002) LTP leads to rapid surface expression of NMDA but not AMPA receptors in adult rat CA1. Nat Neurosci 5:27-33.Wenthold RJ, Prybylowski K, Standley S, Sans N, Petralia RS (2003) Trafficking of NMDA receptors. Annu Rev Pharmacol Toxicol 43:335-358.Zukin RS, Bennett MVL (1995) Alternatively spliced isoforms of the NMDARI receptor subunit. Trends Neurosci 18:306-313.Note: Dr. Michael Browning, a co-author of one of the cited papers, is President and founder of PhosphoSolutions |
| 保存条件： | Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| 标记物： | Prepared from rabbit serum by affinity purification using a column to which the peptide immunogen was coupled. |
| 主要序列号： | P35439 |
| 分子量： | 120 KDa |
| 规格： | 25 µg | 价格： | ¥3685.00 |
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Western blot of 10 ug of HEK 293 cells expressing: Lane 1 - NR1 subunit containing the N1 and C2' Insert showing specific immunolabeling of the ~120k NR1 subunit of the NMDA receptor containing the N1 splice variant insert.; Lane 2 - NR1 subunit containing only the C2 Insert;Lane 3 - NR1 subunit containing the C1 and C2' Insert; Lane 4 - without NR1 expression (Mock).
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