HUVEC(human umbilical vein) whole cell lysate (non-denatured)
- 参考价格： ￥1500.0
- 品 牌： abnova
- 货 号： L055V2
- 宿 主： 人
- 组 织： Human Umbilical Vein
- 描 述： Whole cell lysate (non-denatured)
- 保存条件： Store at -80°C. Aliquot to avoid repeated freezing and thawing.
- 制备方法： Cell lysate was prepared by homogenization in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors (Sigma). Cell debris was removed by centrifugation. Protein concentration was determined with Bradford assay (Bio-Rad protein assay, Microplate Standard assay). The lysate was adjusted to 2 mg/ml.
- 详细信息
- 图片
详细信息
应用与保存
| 保存缓冲液： | In modified RIPA Lysis Buffer. |
其他
| 用途： | Use it directly for immuno-precipitation, or heat lysate with SDS gel loading buffer to 95°C for 5 minutes followed by rapid cooling for western blot application. If dissociating conditions are required, add reducing agent prior to heating. |
| 裂解缓冲液： | Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF. |
| 规格： | 200 ug | 价格： | ￥1500 |
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