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Enterokinase light chain (endopeptidase)
- 参考价格： ￥4020.0
- 品 牌： abnova
- 货 号： P3376
- 应用范围： 功能测定,十二烷基硫酸钠聚丙烯酰胺凝胶电泳
- 宿 主： 人
- 来 源： Porcine
- 保存条件： Store at -80°C. Aliquot to avoid repeated freezing and thawing.
- 保存缓冲液： In 20 mM Tris-HCl, 200 mM NaCl, 2 mM CaCl 2 , 50% glycerol, pH 7.2
- 详细信息
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详细信息
产品特性
| 纯度： | > 95% |
其他
| 描述： | Enhanced recombinant enterokinase light chain with 6 histidines expressed from modified human kidney cell line. Enterokinase (EK) is a specific protease that cleaves a protein after lysine residue at its recognition site: Asp-Asp-Asp-Asp-Lys. Enterokinase will not work if the recognition site is followed by proline. The rEKL x6 contains 6X His tags and can be removed from the cleavage reaction mixture by filtering through Nickel Cadmium column. |
| 生物学活性： | 1 U of rEKL x10 can cleave 95% of 50 ug of fusion protein with enterokinase cleavage site at 25°C after 16 hours of digestion in a buffer containing 25 mM Tris-HCl, 50 mM NaCl, 2 mM CaCl 2 pH 8.0. |
| 规格： | 100 Units | 价格： | ￥4020 |
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