诊断试剂盒中试剂等组分的稳定性对于商业化的检测至关重要。现今稳定剂不仅可以延长保质期,而且可以提高检测试剂盒的可靠性。以 SARS-CoV- 2 抗原为例,我们将解释商业化稳定剂如何在优化工作流程的同时提高分析质量。 Figure 1: Calibration curves of an ELISA that was either blocked only with BSA (left panel) or blocked with BSA and additionally stabilized with Liquid Plate Sealer® (right panel). Calibration curves at the starting day are identical. After 84 days at 37℃, no remaining binding activity of the capture antibodies is detectable in the unstabilized plate. In contrast, the stabilized plate does not show a significant decrease in sensitivity even after 84 days of incubation at 37℃ Figure 2: High-speed ELISA plate production with Liquid Plate Sealer® as blocking reagent and coating stabilizer in a single process step is suitable for sandwich ELISA and competitive tests. In serological assay formats however, it is sometimes better to use a more classical process as shown in the first row. In any case, washing after blocking can be commonly skipped when using Liquid Plate Sealer®.
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