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羧甲基-茯苓聚糖--上海熹垣生物优势提供 中文名:羧甲基-茯苓聚糖 英文名:CM-Pachyman 结构式: High purity CM-Pachyman for use in research, biochemical enzyme assays and in vitro diagnostic analysis. Carboxymethylated, (DS ~ 0.2) highly purified pachyman. A soluble/gelatinous substrate for the assay of endo-1,3-β-D-glucanase. CAS NO. 69552-83-6 PREPARATION: Carboxymethyl pachyman (CM-Pachyman) is prepared by carboxymethylation of highly purified pachyman with chloroacetic acid. Pachyman is a polymer of 1,3-b-linked D-glucosyl residues. PROPERTIES OF CM-PACHYMAN: Degree of carboxymethylation (DS): ~ 0.2. Colour: tan coloured powder. Solubility: Forms a colloidal suspension in water at 0.5 % w/v. Enzyme susceptibility: Readily hydrolysed by endo-1,3-b-glucanase. DISSOLUTION: Gradually add 0.5 g of CM-pachyman to 90 mL of vigorously stirring water at 90°C. Continue stirring for approx. 1 h (until the polysaccharide is completely dispersed). Cool the solution to room temperature and add 5 mL of sodium acetate buffer (2 M, pH 5.0). Adjust the volume to 100 mL and store the solution in a well sealed glass container at 4°C. Prevent microbial infection by adding a few drops of toluene to the storage bottle. For some 1,3-b-glucanases, a higher pH is require for activity; in these cases, substitute an appropiate buffer for the acetate buffer (eg. MOPS) 更多产品信息,请联系我们! 上海熹垣生物科技有限公司 固话:021-58116080 商城:http://xiyuan-bio.biomart.cn |