手机验证
/
PA-TU-8988S; PaTu8988s; PaTu8988S; PATU8988S; PaTu 8988 S; PaTu-8988s; PATU-8988S; PATU-S; PA-TU S
/
充足
/
/
/
/
/
胰腺腺癌
人胰腺癌细胞
人
/
上皮样细胞和成纤维样细胞均有
/
胰腺腺癌
复苏发货或干冰冷冻发货
/
贴壁
1 x 10^6 cells/vial/T25
细胞名称:PATU8988人胰腺癌细胞(STR鉴定正确)
产品货号:C1390
培养条件:MEM+5%FBS+1%PS(货号:M0304)
生长特性:贴壁
细胞形态:上皮样
产品规格:1 x 10^6 cells/vial/T25
产品货号 | 细胞名称 | 中文名称 | 生长特性 | 细胞形态 | 产品规格 |
C1004 | PANC-1 | 人胰腺癌细胞 | 贴壁 | 上皮样 | 1 x 10^6 cells/vial/T25 |
C1023 | BxPC-3 | 人原位胰腺腺癌细胞 | 贴壁 | 上皮样 | 1 x 10^6 cells/vial/T25 |
C1086 | HPAF-II | 人胰腺癌细胞 | 贴壁 | 上皮样 | 1 x 10^6 cells/vial/T25 |
C1155 | AsPC-1 | 人转移胰腺腺癌细胞 | 贴壁 | 上皮样 | 1 x 10^6 cells/vial/T25 |
C1157 | MIA PaCa-2 | 人胰腺癌细胞 | 贴壁 | 上皮样 | 1 x 10^6 cells/vial/T25 |
C1188 | hTERT-HPNE | 人正常胰腺导管细胞 | 贴壁 | 上皮样 | 1 x 10^6 cells/vial/T25 |
C1196 | SW 1990 | 人胰腺癌细胞系 | 贴壁 | 上皮样 | 1 x 10^6 cells/vial/T25 |
C1206 | CFPAC-1 | 人胰腺癌细胞 | 贴壁 | 上皮样 | 1 x 10^6 cells/vial/T25 |
C1248 | HPDE6-C7 | 人正常胰腺导管上皮细胞系 | 贴壁 | 上皮样 | 1 x 10^6 cells/vial/T25 |
C1333 | 1.1B4/PANC-1 | 人胰腺癌细胞 | 贴壁 | 上皮样 | 1 x 10^6 cells/vial/T25 |
用户须知:
1、关于货期:除复苏细胞外,承诺货期1周内到(节假日顺延),大多数产品1-3天内发货。细胞产品因需要复苏后进行1-2代传代调整后等待细胞长满,期间进行微生物检测,货期1、2周左右。
2、关于订购:为了保证您的权益,下单前务必联系我司客户服务热线(021-55150076)。
细胞传代:
1.弃去培养液,用PBS洗涤1-2次;
2.加入1.0ml胰酶消化液,37℃消化,显微镜下观察细胞消化情况,若细胞回缩变圆、透亮、轻拍甁壁呈流沙样脱落,则迅速拿回操作台,加入完全培养液,终止消化并轻轻吹打细胞,使其变成单细胞悬液;
3.将细胞收集于离心管中离心1000rmp/5min,弃上清,轻弹管底,将细胞弹散;
4.加入新鲜培养液重悬细胞,进行传代、冻存;
5.如果没有特别说明,建议收到细胞后的第一次传代比例为1:2。
6.注意培养基PH值变化,定期换液(每周2-3次)。
安全性:
所有肿瘤和病毒转染的细胞均视为有潜在的生物危害性,必须在二级生物安全台内操作,并请注意防护。
联系人:胡经理
电话:15921028707(微信同号)
:3407506161
:021-55150076
官网:https://whelab.com
公司邮箱:info@whelab.com
技术邮箱:tech@whelab.com
地址:上海宝山区呼兰西路129号8号楼4层
风险提示:丁香通仅作为第三方平台,为商家信息发布提供平台空间。用户咨询产品时请注意保护个人信息及财产安全,合理判断,谨慎选购商品,商家和用户对交易行为负责。对于医疗器械类产品,请先查证核实企业经营资质和医疗器械产品注册证情况。
PubMed=1348891; DOI=10.1007/BF02890431
Elsasser H.-P., Lehr U., Agricola B., Kern H.F.
Establishment and characterisation of two cell lines with different grade of differentiation derived from one primary human pancreatic adenocarcinoma.
Virchows Arch. B. Cell. Pathol. Incl. Mol. Pathol. 61:295-306(1992)
PubMed=8194712; DOI=10.1016/0016-5085(94)90422-7
Simon B., Weinel R., Hohne M., Watz J., Schmidt J., Kortner G., Arnold R.
Frequent alterations of the tumor suppressor genes p53 and DCC in human pancreatic carcinoma.
Gastroenterology 106:1645-1651(1994)
PubMed=11668190; DOI=10.1177/002215540104901105
Quentmeier H., Osborn M., Reinhardt J., Zaborski M., Drexler H.G.
Immunocytochemical analysis of cell lines derived from solid tumors.
J. Histochem. Cytochem. 49:1369-1378(2001)
PubMed=15126341; DOI=10.1158/0008-5472.CAN-03-3159
Heidenblad M., Schoenmakers E.F.P.M., Jonson T., Gorunova L., Veltman J.A., van Kessel A.G., Hoglund M.
Genome-wide array-based comparative genomic hybridization reveals multiple amplification targets and novel homozygous deletions in pancreatic carcinoma cell lines.
Cancer Res. 64:3052-3059(2004)
PubMed=15688027; DOI=10.1038/sj.onc.1208383
Heidenblad M., Lindgren D., Veltman J.A., Jonson T., Mahlamaki E.H., Gorunova L., van Kessel A.G., Schoenmakers E.F.P.M., Hoglund M.
Microarray analyses reveal strong influence of DNA copy number alterations on the transcriptional patterns in pancreatic cancer: implications for the interpretation of genomic amplifications.
Oncogene 24:1794-1801(2005)
PubMed=22460905; DOI=10.1038/nature11003
Barretina J.G., Caponigro G., Stransky N., Venkatesan K., Margolin A.A., Kim S., Wilson C.J., Lehar J., Kryukov G.V., Sonkin D., Reddy A., Liu M., Murray L., Berger M.F., Monahan J.E., Morais P., Meltzer J., Korejwa A., Jane-Valbuena J., Mapa F.A., Thibault J., Bric-Furlong E., Raman P., Shipway A., Engels I.H., Cheng J., Yu G.-Y.K., Yu J.-J., Aspesi P. Jr., de Silva M., Jagtap K., Jones M.D., Wang L., Hatton C., Palescandolo E., Gupta S., Mahan S., Sougnez C., Onofrio R.C., Liefeld T., MacConaill L.E., Winckler W., Reich M., Li N.-X., Mesirov J.P., Gabriel S.B., Getz G., Ardlie K., Chan V., Myer V.E., Weber B.L., Porter J., Warmuth M., Finan P., Harris J.L., Meyerson M.L., Golub T.R., Morrissey M.P., Sellers W.R., Schlegel R., Garraway L.A.
The Cancer Cell Line Encyclopedia enables predictive modelling of anticancer drug sensitivity.
Nature 483:603-607(2012)
PubMed=22585861; DOI=10.1158/2159-8290.CD-11-0224
Marcotte R., Brown K.R., Suarez Saiz F.J., Sayad A., Karamboulas K., Krzyzanowski P.M., Sircoulomb F., Medrano M., Fedyshyn Y., Koh J.L.Y., van Dyk D., Fedyshyn B., Luhova M., Brito G.C., Vizeacoumar F.J., Vizeacoumar F.S., Datti A., Kasimer D., Buzina A., Mero P., Misquitta C., Normand J., Haider M., Ketela T., Wrana J.L., Rottapel R., Neel B.G., Moffat J.
Essential gene profiles in breast, pancreatic, and ovarian cancer cells.
Cancer Discov. 2:172-189(2012)
DOI=10.4172/2324-9293.1000104
Wagenhauser M.U., Ruckert F., Niedergethmann M., Grutzmann R., Saeger H.-D.
Distribution of characteristic mutations in native ductal adenocarcinoma of the pancreas and pancreatic cancer cell lines.
Cell Biol. Res. Ther. 2:1000104.1-1000104.5(2013)
PubMed=25167228; DOI=10.1038/bjc.2014.475
Hamidi H., Lu M., Chau K., Anderson L., Fejzo M., Ginther C., Linnartz R., Zubel A., Slamon D.J., Finn R.S.
KRAS mutational subtype and copy number predict in vitro response of human pancreatic cancer cell lines to MEK inhibition.
Br. J. Cancer 111:1788-1801(2014)
PubMed=25485619; DOI=10.1038/nbt.3080
Klijn C., Durinck S., Stawiski E.W., Haverty P.M., Jiang Z.-S., Liu H.-B., Degenhardt J., Mayba O., Gnad F., Liu J.-F., Pau G., Reeder J., Cao Y., Mukhyala K., Selvaraj S.K., Yu M.-M., Zynda G.J., Brauer M.J., Wu T.D., Gentleman R.C., Manning G., Yauch R.L., Bourgon R., Stokoe D., Modrusan Z., Neve R.M., de Sauvage F.J., Settleman J., Seshagiri S., Zhang Z.-M.
A comprehensive transcriptional portrait of human cancer cell lines.
Nat. Biotechnol. 33:306-312(2015)
PubMed=26216984; DOI=10.1073/pnas.1501605112
Daemen A., Peterson D., Sahu N., McCord R., Du X.-N., Liu B., Kowanetz K., Hong R., Moffat J., Gao M., Boudreau A., Mroue R., Corson L., O'Brien T., Qing J., Sampath D., Merchant M., Yauch R.L., Manning G., Settleman J., Hatzivassiliou G., Evangelista M.
Metabolite profiling stratifies pancreatic ductal adenocarcinomas into subtypes with distinct sensitivities to metabolic inhibitors.
Proc. Natl. Acad. Sci. U.S.A. 112:E4410-E4417(2015)
PubMed=26589293; DOI=10.1186/s13073-015-0240-5
Scholtalbers J., Boegel S., Bukur T., Byl M., Goerges S., Sorn P., Loewer M., Sahin U., Castle J.C.
TCLP: an online cancer cell line catalogue integrating HLA type, predicted neo-epitopes, virus and gene expression.
Genome Med. 7:118.1-118.7(2015)
PubMed=28196595; DOI=10.1016/j.ccell.2017.01.005
Li J., Zhao W., Akbani R., Liu W.-B., Ju Z.-L., Ling S.-Y., Vellano C.P., Roebuck P., Yu Q.-H., Eterovic A.K., Byers L.A., Davies M.A., Deng W.-L., Gopal Y.N.V., Chen G., von Euw E.M., Slamon D.J., Conklin D., Heymach J.V., Gazdar A.F., Minna J.D., Myers J.N., Lu Y.-L., Mills G.B., Liang H.
Characterization of human cancer cell lines by reverse-phase protein arrays.
Cancer Cell 31:225-239(2017)
PubMed=30894373; DOI=10.1158/0008-5472.CAN-18-2747
Dutil J., Chen Z.-H., Monteiro A.N.A., Teer J.K., Eschrich S.A.
An interactive resource to probe genetic diversity and estimated ancestry in cancer cell lines.
Cancer Res. 79:1263-1273(2019)
PubMed=31068700; DOI=10.1038/s41586-019-1186-3
Ghandi M., Huang F.W., Jane-Valbuena J., Kryukov G.V., Lo C.C., McDonald E.R. III, Barretina J.G., Gelfand E.T., Bielski C.M., Li H., Hu K., Andreev-Drakhlin A.Y., Kim J., Hess J.M., Haas B.J., Aguet F., Weir B.A., Rothberg M.V., Paolella B.R., Lawrence M.S., Akbani R., Lu Y., Tiv H.L., Gokhale P.C., de Weck A., Mansour A.A., Oh C., Shih J., Hadi K., Rosen Y., Bistline J., Venkatesan K., Reddy A., Sonkin D., Liu M., Lehar J., Korn J.M., Porter D.A., Jones M.D., Golji J., Caponigro G., Taylor J.E., Dunning C.M., Creech A.L., Warren A.C., McFarland J.M., Zamanighomi M., Kauffmann A., Stransky N., Imielinski M., Maruvka Y.E., Cherniack A.D., Tsherniak A., Vazquez F., Jaffe J.D., Lane A.A., Weinstock D.M., Johannessen C.M., Morrissey M.P., Stegmeier F., Schlegel R., Hahn W.C., Getz G., Mills G.B., Boehm J.S., Golub T.R., Garraway L.A., Sellers W.R.
Next-generation characterization of the Cancer Cell Line Encyclopedia.
Nature 569:503-508(2019)
PubMed=31395879; DOI=10.1038/s41467-019-11415-2
Yu K., Chen B., Aran D., Charalel J., Yau C., Wolf D.M., van 't Veer L.J., Butte A.J., Goldstein T., Sirota M.
Comprehensive transcriptomic analysis of cell lines as models of primary tumors across 22 tumor types.
Nat. Commun. 10:3574.1-3574.11(2019)
需要更多技术资料 索取更多技术资料