Microglia Medium (MM) is a complete medium designed for optimal growth of normal human brain‐derived microglia in vitro. It is a sterile, liquid medium which contains essential and non‐essential amino acids, vitamins, organic and inorganic compounds, hormones, growth factors, trace minerals and a 5% concentration of fetal bovine serum. The medium is bicarbonate buffered and has a pH of 7.4 when equilibrated in an incubator with an atmosphere of 5% CO2/95% air. The medium is formulated (quantitatively and qualitatively) to provide a defined and optimally balanced nutritional environment that selectively promotes growth of normal human microglia in vitro.
小胶质细胞培养基(MM)是一种为体外生长的正常人脑衍生小胶质细胞而设计的完整培养基。它是一种无菌的液体培养基,含有必需氨基酸和非必需氨基酸、维生素、有机和无机化合物、激素、生长因子、微量矿物质和5%浓度的胎牛血清。培养基经过碳酸氢盐缓冲,在5%二氧化碳/95%空气的培养箱中进行平衡时pH值为7.4。培养基的配方(定量和定性)是为了提供一个明确的和最佳平衡的营养环境,选择性地促进体外正常人类小胶质细胞的生长。