Mesenchymal Stem Progeitors are derived from human embryonic cell line, H9 (WA09). Cells are >80% CD90, CD105, CD166 and CD73 positive and <5% Oct4 positive. Cells have the capabilties to: Form adherent monolayers – ideal for 96- and 384-well formats for high throughput and high content cell-based assays.To differentiate into a wide variety of mesenchymal subtypes, including osteogenic and chondrogenic lineages (but not adipogenic)– ideal for drug discovery for a wide variety of biological targets and basic research in bone, cartilage, metabolic and immunological diseases.Passage up to 10X.Note: Please include a 30 USD dry ice charge when ordering. Pricing is for US and Canada only.Specifications: Cell Type: mesenchymal progenitor cells Species: human Source: derived from human embryonic cell line, H9 (WA09) Morphology: fibroblasticQuantity: >1x10^6 cells per vial (viable post-thaw) Shipping method: dry iceStorage: liquid nitrogenGrowth Properties: adherent, proliferative Medium: αMEM Medium supplemented with 10% FBS, Pen/Strep and L-glutamine Phenotyping: Cells are >80% CD90, CD105, CD166 and CD73 positive and <5% Oct4 positive. DNA fingerprint: Results are consistent with the presence of a single cell line with no evidence of culture cross contamination. Pathogen testing: This lot was derived from the H9 hESC line that has been tested for Hepatitis B, Hepatitis C, HIV-1, HIV-2, HTLV-I/II, HSV1, HSV2, EBV, and CMV. The H9 cell line has been tested and shown to be negative (performed by GIVF Laboratories). Mesenchymal cells are also tested for sterility and mycoplasma contamination.间充质干细胞祖细胞来源于人胚胎细胞系H9 (WA09)。细胞中CD90、CD105、CD166、CD73阳性占80%,lt阳性占5%,Oct4阳性。细胞有能力形成适合96井和384井格式的贴壁单分子层,用于高通量和高含量的细胞分析。分化为多种间充质亚型,包括成骨和软骨源性谱系(但非脂肪源性),是骨骼、软骨、代谢和免疫疾病各种生物靶点和基础研究的理想药物发现。通道长达10X。注:请在订购时包括30美元的干冰费用。价格仅针对美国和加拿大。规格:细胞类型:物种间叶细胞祖细胞:人类来源:来自人类胚胎细胞系,H9 (WA09)形态:成纤维细胞的数量:和gt; 1 x10 ^ 6细胞/瓶(可行post-thaw)运输方法:干冰保存方法:液态氮增长属性:附着、增殖培养基:αMEM培养基补充10%的边后卫,笔/喉炎和谷酰胺表现型:细胞和gt; 80% CD90、CD105,存在和CD73积极,lt; 5% Oct4积极。DNA指纹图谱:结果与单细胞的存在一致,没有培养交叉污染的证据。病原体检测:该批次来自H9 hESC细胞系,已对乙型肝炎、丙型肝炎、HIV-1、HIV-2、HTLV-I/II、HSV1、HSV2、EBV和CMV进行检测。H9细胞系已经过测试,显示为阴性(由GIVF实验室进行)。还测试间充质细胞的无菌性和支原体污染。